Single Donor Platelet, Transfusion

CERNER / EPIC MNEMONIC: PLPH
POE DESCRIPTION:

PLTS (Pheresis/donor ~ 250 mL)

 

TURNAROUND TIME: 20 minutes (if product is in-house)
SPECIAL INSTRUCTIONS: HLA matched platelets require 2 days advance notice and special arrangements with an outside center.
CONTAINER TYPE: Pink top tube
COLLECTION: Special Labeling Requirements: The content of each sample label must include the patient's full name and medical record number, the phlebotomist's initials, the date and time the sample was collected and, for non-LLT draws, the initials of a second medical professional who has confirmed that the sample came from the patient whose name is on the label; the computer label must have this same information plus an accession number and the tests ordered. NOTE:  LLT phlebotomists should write 'LLT' for the second set of initials.
SPECIMEN REQUIREMENTS: Blood
MINIMUM VOLUME: 7 mL
HANDLING INSTRUCTIONS: Special arrangements and sample requirements for HLA matched platelets.
REFERENCE RANGE: Each unit contains at least 3 x 10¹¹ platelets.
TEST SYNONYM(S):Plateletpheresis; HLA Matched Platelets

Special Antigen Typing

CERNER / EPIC MNEMONIC: AG
CPT CODE: 86903
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: 2 hours
CONTAINER TYPE:

 

Pink top tube

COLLECTION: Special Labeling Requirements: The content of each sample label must include the patient's full name and medical record number, the phlebotomist's initials, the date and time the sample was collected and, for non-LLT draws, the initials of a second medical professional who has confirmed that the sample came from the patient whose name is on the label; the computer label must have this same information plus an accession number and the tests ordered. NOTE:  LLT phlebotomists should write 'LLT' for the second set of initials.
SPECIMEN REQUIREMENTS: Blood
MINIMUM VOLUME: 7 mL

Salicylate, Serum

CERNER / EPIC MNEMONIC: SAL
POE DESCRIPTION: SALICYLATE; ASPIRIN
CPT CODE: 80196
DAYS PERFORMED: Specimen accepted daily, 24 hours
TURNAROUND TIME: Routine: 8 hours; stat: 1 hour
CONTAINER TYPE:

Red top tube or SST tube

SPECIMEN REQUIREMENTS: Blood (serum)
MINIMUM VOLUME: 0.4 mL blood (0.2 mL serum)
REFERENCE RANGE: Therapeutic: 10-20 mg/dL
CRITICAL VALUES: >40 mg/dL
LAST UPDATED:

10-3-2013

TEST SYNONYM(S):Acetysalicylic Acid, Blood; ASA, Blood; Aspirin, Blood; Salicylic Acid, Blood

Semen Analysis

CERNER / EPIC MNEMONIC: SEMEN ANAL
POE DESCRIPTION:

SEMEN ANALYSIS WITH MORPHOLOGY

CPT CODE: 89320
DAYS PERFORMED:

Monday-Friday, 0700 am - 1100 am with appointment

TURNAROUND TIME:

3-5 business days

SPECIAL INSTRUCTIONS:

Call office to schedule appointments.  Appointment should be scheduled at least 3 days in advance.  You must receive a Medical Record Number in Room 104 Pro Bldg I prior to producing semen specimen.  Semen specimens are produced in the Andrololgy Lab, Suite 119 Pro Bldg I.  Please allow 2-3 days of sexual abstinence prior to specimen collection.

CONTAINER TYPE:

Sterile specimen container (provided by lab)

SPECIMEN REQUIREMENTS:

Semen

REJECTION CRITERIA: Improper collection, incomplete requisition information
MINIMUM VOLUME:

1.0

HANDLING INSTRUCTIONS:

Specimen should ideally be produced in lab.

REFERENCE RANGE:

On form

LAST UPDATED:

9-5-2013

Sickle Cell Test

CERNER / EPIC MNEMONIC: SICKLE SOL
POE DESCRIPTION: SICKLE CELL SOLUBILITY TEST; SICKLE SOL
CPT CODE: 85660
DAYS PERFORMED: Daily, 24 hours, available stat
TURNAROUND TIME: 8 hours
CONTAINER TYPE:

Lavender top

SPECIMEN REQUIREMENTS: Blood
MINIMUM VOLUME: 1.5 mL venous, 250 µL capillary whole blood
REFERENCE RANGE: Negative
TEST SYNONYM(S):Sickle Cell Screen; Sickle Prep

Smear for Morphology

CERNER / EPIC MNEMONIC: MORPH SM
POE DESCRIPTION: MORPHOLOGY SMEAR FOR HEMATOLOGY
CPT CODE: 85007
DAYS PERFORMED: Daily, 24 hours; available stat
TURNAROUND TIME: 8 hours
SPECIAL INSTRUCTIONS: Same specimen must have a CBC request also.┐ Not for general morphology but for specific morphological abnormality (i.e. spherocytes or cell inclusions).
CONTAINER TYPE: Lavender top (EDTA) tube
SPECIMEN REQUIREMENTS: Blood
REJECTION CRITERIA: Specimen clotted, >24 hours old
MINIMUM VOLUME: 1.5 mL venous, 250 µL capillary whole blood
HANDLING INSTRUCTIONS: Specimen should be sent to the laboratory as soon as possible.
REFERENCE RANGE: None seen
TEST SYNONYM(S):Morphology Smear

Sodium, Blood

CERNER / EPIC MNEMONIC: NA; BG/NA
POE DESCRIPTION: SODIUM; NA
CPT CODE: 84295
DAYS PERFORMED:

Daily

TURNAROUND TIME: Routine: 8 hours; Blood Gas Laboratory: 15 minutes; stat: 1 hour
SPECIAL INSTRUCTIONS: Deliver immediately on ice if ordered with blood gases or ionized Ca++
CONTAINER TYPE:

SST tube, heparinized syringe (Blood Gas Laboratory)

SPECIMEN REQUIREMENTS: Blood (serum, plasma)
MINIMUM VOLUME: 0.4 mL blood (0.2 mL serum, 1 mL plasma)
REFERENCE RANGE: 137-147 mmol/L
CRITICAL VALUES: <120 >160
TEST SYNONYM(S):Na

Sodium, Fluid or Cerebrospinal Fluid

CERNER / EPIC MNEMONIC: F/NA
POE DESCRIPTION: FLUID SODIUM
CPT CODE: 84302
DAYS PERFORMED: Specimen accepted daily, 24 hours
TURNAROUND TIME: 1 hour
SPECIAL INSTRUCTIONS: Must specify fluid type on the requisition.
CONTAINER TYPE:

Red top tube - 10 mLRed top tube - 4 mLSterile 80 mL specimen container

SPECIMEN REQUIREMENTS: Body fluid
MINIMUM VOLUME: 0.2 mL
REFERENCE RANGE: None established
TEST SYNONYM(S):Fluid Sodium

Sodium, Quantitative, Urine

CERNER / EPIC MNEMONIC: RU/NA (random urine); U/NA (24-hour urine)
POE DESCRIPTION:

Urine Sodium Random; Urine Sodium Timed

CPT CODE: Random = 84300; Timed = 84300, 81050
DAYS PERFORMED: Specimen accepted daily, 24 hours
TURNAROUND TIME: Stat: 1 hour (random urine only); Routine: 8 hours
SPECIAL INSTRUCTIONS: For timed urine collection, instruct the patient to void at the beginning of the collection period and discard the specimen. Then collect all urine including the final specimen voided at the end of the collection period. Containers must be labeled with patient`s full name, room number, date and time collection started, and date and time collection finished.
CONTAINER TYPE:

Random Sterile 80 ml specimen container; Timed 24 hour urine container (Bottle #1) with no preservative

SPECIMEN REQUIREMENTS:

Random or 24-hour urine

MINIMUM VOLUME: Random urine: 1 mL; Timed urine: Submit entire urine collection to the laboratory.
HANDLING INSTRUCTIONS: Refrigerate during collection.
REFERENCE RANGE: Random urine: None established; Timed urine: Newborn to 1 year: 0-4 mmol/24 hours; 1-15 years: 40-180 mmol/24 hours; 15 years to adult: 80-200 mmol/24 hours
TEST SYNONYM(S):Na, Urine; Urine Na; Urine Sodium

Specific Gravity, Fluid

CERNER / EPIC MNEMONIC: F/SPGR
POE DESCRIPTION: FL SPECIFIC GRAVITY
CPT CODE: 84315
DAYS PERFORMED: Specimen accepted daily
TURNAROUND TIME: 8 hours
SPECIAL INSTRUCTIONS: Fluid type must be specified on the requisition.
CONTAINER TYPE: Random urine container
SPECIMEN REQUIREMENTS: Body fluid
MINIMUM VOLUME: 0.2 mL
REFERENCE RANGE: None established; must be interpreted with clinical findings.
TEST SYNONYM(S):Fluid Specific Gravity

Spinal Fluid Cytology

CERNER / EPIC MNEMONIC: (call Pathology)
CPT CODE: 88104
DAYS PERFORMED: Mon-Fri, 0730-1700
TURNAROUND TIME: Immediate evaluation available; 24 hours for routine evaluation
SPECIAL INSTRUCTIONS: Specify specimen origin. Clinical diagnosis and clinical history must be indicated on requisition.
CONTAINER TYPE: Sterile tube from lumbar puncture tray or sterile disposable container
SPECIMEN REQUIREMENTS: Fresh fluid
MINIMUM VOLUME: 1-2 mL
HANDLING INSTRUCTIONS: Specimens should be transported to Rm 470 Jelke after collection.
TEST SYNONYM(S):Cerebrospinal Fluid Cytology

Sputum Cytology

CERNER / EPIC MNEMONIC: (call Pathology)
CPT CODE: 88104
DAYS PERFORMED: Mon-Fri, 0730-1700
TURNAROUND TIME: 24-48 hours
SPECIAL INSTRUCTIONS: Clinical diagnosis and clinical history must be indicated on requisition.
CONTAINER TYPE: Sterile specimen container
SPECIMEN REQUIREMENTS: Expectorated sputum, not saliva or nasal aspirates
MINIMUM VOLUME: 3 mL (1 tablespoon)
HANDLING INSTRUCTIONS: Refrigerate specimens until transported to the laboratory.

Schlichter Test

CERNER / EPIC MNEMONIC: SERUM KILL
CPT CODE: 87197 (each)
CDM NUMBER: 3421057 (each)
TEST INFORMATION: This test is used to determine the maximum dilution of the serum or body fluid, MBD, which is bacteridical for the patient`s infecting bacterium; monitor total therapeutic effect.
DAYS PERFORMED: Monday-Thursday
TURNAROUND TIME: 2-5 days
SPECIAL INSTRUCTIONS: If a Schlichter test is desired, the physician must request that the laboratory save the patient`s isolate (if from other than a sterile body site) within 7 days of submission of specimen for initial culture. If the isolate has not been saved, the test cannot be performed. Requisition MUST state all current antibiotic therapy. Time of specimen collection should be indicated on requisition.
CONTAINER TYPE: Red topVacutainer tube
COLLECTION: Specimen should be collected just before or within 15 minutes of the next antibiotic dose to obtain a TROUGH level. Specimen should be collected within 15-30 minutes after the end of an IV infusion, 45-60 minutes after an IM injection, or 90 minutes after oral intake to obtain a PEAK level.
SPECIMEN REQUIREMENTS: Blood (serum), bacterial isolate causing infection
REJECTION CRITERIA: Blood: excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume, specimen submitted in an SST tube. Isolate: isolate not saved, isolate non-viable.
MINIMUM VOLUME: 2 mL serum
HANDLING INSTRUCTIONS: The blood specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: Generally, the peak bactericidal activity should be observed at >1:8 dilution.
LIMITATIONS OF TEST: Results will reflect the combined in-vitro effect of all antimicrobial agents present in the patient`s serum or body fluid on the infecting organism(s). Results are accurate to plus or minus one dilution. Serum inhibitory dilution will be reported. A serum inhibitory titer might suggest an adequate therapeutic level, but would give no clue to potential toxicity, when an extremely narrow margin exists between a therapeutically adequate dose and a possibly toxic one(i.e. aminoglycosides).
METHODOLOGY: Serial tube dilution.
REFERRAL LABORATORY:

Commercial laboratory

TEST SYNONYM(S):Serum Cidal Test

Sjogren`s Antibodies (Anti-ENA)

CERNER / EPIC MNEMONIC: SSA /B
POE DESCRIPTION: SJOGRENS ANTIBODIES; SSA/B
CPT CODE: 86235 (x2)
TEST INFORMATION:

 

DAYS PERFORMED:

Wednesday

TURNAROUND TIME: 1-7 days
CONTAINER TYPE:

Red top Vacutainer® tube or SST tube

SPECIMEN REQUIREMENTS:

4 mL blood (2 mL serum)

REJECTION CRITERIA: Hemolysis, gross contamination, icteria, lipemic
MINIMUM VOLUME: 2 mL blood (1.0 mL serum)
HANDLING INSTRUCTIONS:

 

REFERENCE RANGE:

SSA:  0.00-0.89 Index Value

SSB:  0.00-0.89 Index Value

METHODOLOGY: Enzyme immunoassay (EIA)
ADDITIONAL INFORMATION:

 

TEST SYNONYM(S):SS-A and SS-B Antibodies; RO AB; LA AB; SJOG AB; ANTI-LA; ANTI-RO

Skin Culture, Fungus

CERNER / EPIC MNEMONIC:

C SFUNGUSSM; C SFUNGUS (culture) or C SFUNSM (cult with smear)

POE DESCRIPTION: CU FUNGUS SMEAR (SKIN ONLY); C SFUNSM
CPT CODE: 87101; 87206
TEST INFORMATION: Test includes a culture for fungi and performance of a direct fungal smear. All fungal isolates will be identified. Other isolated organisms (i.e. aerobes, mycobacteria, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED:

Daily, 24 hours

TURNAROUND TIME: Smear results: same day if the specimen is received in the laboratory no later than 1400. Preliminary culture reports are available after 1 week or when a fungal isolate is detected. Cultures will be finalized after 4 weeks.
SPECIAL INSTRUCTIONS: The specific anatomic site of the specimen MUST be specified on the requisition or order with surface and/or deep or surgical biopsies delineated.
CONTAINER TYPE: Sealed sterile container, no preservative; sterile Petri dish (available from Clinical Microbiology Laboratory)
COLLECTION: Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface organisms. Infected hair should be cut with sterile scissors or plucked with sterile forceps and placed in a sealed, sterile container. A Wood`s lamp is useful in the collection of specimens in tinea capitis infections, since hairs infected by most members of the genus Microsporum exhibit fluorescence under a Wood`s lamp. If diseased hair stubs or fluorescent hairs are not apparent, scrape the edge of a scalp lesion with a sterile scalpel. Infected nails should be sampled from beneath the nail to obtain softened material from the nail bed. If unable to obtain adequate material from the nail bed, a scalpel should be utilized to scrape the superficial portions of the nail obtaining subsurface material. Nail clippings are unacceptable. When obtaining a cutaneous specimen, the area to be sampled should first be cleansed with 70% alcohol to remove bacterial contaminants. Typical ''ringworm'' lesions should be sampled from the erythematous, peripheral growing margin.
SPECIMEN REQUIREMENTS: Hair, nail scrapings, skin scrapings
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, specimen received in a preservative, nail clippings.
MINIMUM VOLUME: As much as possible
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE: No fungi observed/isolated
LIMITATIONS OF TEST: The Calcofluor white stain demonstrates yeast forms, spores, and the hyphae of fungi. The filaments of Nocardia species, Streptomyces species, and Actinomyces species, as well as, the capsule of Cryptococcus species are NOT satisfactorily demonstrated with this methodology.
METHODOLOGY: Fungal smear: calcofluor white stain. Culture: Conventional culture utilizing media specifically formulated for fungal isolation.
LAST UPDATED:

5-27-2014

TEST SYNONYM(S):Fungus Culture, Skin

Skin Culture, Mycobacterium

CERNER / EPIC MNEMONIC: C AFBSM
POE DESCRIPTION: CU MYCOBACTERIUM W SMEAR; CU AFB AND SMEAR; C AFBSM
CPT CODE: 87117; 87206
TEST INFORMATION: Test includes culture and acid-fast stain (if specimen volume is adequate). Culture includes decontamination and concentration. If a mycobacterial species is isolated, the organism will be definitely identified. Susceptibility testing will be performed depending upon the isolate identification. Other isolated organisms (i.e. aerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily
TURNAROUND TIME: Smear results: 24 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 1 week or when a mycobacterial isolate is detected. Negative cultures with negative smears will be finalized after 8 weeks. Negative cultures with positive smears will be finalized after 12 weeks. Completion of culture reports may require additional time when mycobacteria are isolated and/or susceptibility testing is performed.
SPECIAL INSTRUCTIONS: The specific anatomic site of the specimen MUST be specified on the order or the requisition.
CONTAINER TYPE: Sealed sterile container, no preservative
COLLECTION: Specimens are to be collected from a site prepared utilizing aseptic technique. The area to be sampled should first be cleansed with 70% alcohol to remove bacterial contaminants.
SPECIMEN REQUIREMENTS: Skin biopsy, subcutaneous material, surgical tissue
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative. Only three specimens per body site with negative acid-fast smears will be processed. Subsequent specimens will be rejected. Specimens from known positive patients will be accepted only once a week for culture and smear per body site.
MINIMUM VOLUME: As much as possible
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate specimen.
REFERENCE RANGE: No acid-fast bacilli observed on smear or isolated in culture
LIMITATIONS OF TEST: Mycobacterium leprae CANNOT be grown in culture. A single negative smear does not rule out the presence of mycobacterial infection.
METHODOLOGY: Acid-fast smear: fluorochrome stain. Culture: inoculation of conventional culture media at two temperatures and a rapid medium for automated continuous analysis.

ADDITIONAL INFORMATION:

Mycobacterium marinum is frequently responsible for granulomatous cutaneous lesions acquired from heated swimming pools and fish tanks. Lesions similar to those seen with sporotrichosis follow lymphatics. M. fortuitum and M. chelonae complex organisms are saprophytic mycobacteria which can cause cutaneous abscesses and osteomyelitis in traumatic injury. M. ulcerans causes a chronic granulomatous skin lesion called Buruli ulcer. M. ulcerans may also be saprophytic, colonizing cutaneous ulcers associated with circulatory insufficiency and diabetes. M. ulcerans is uncommon in North America; it is most frequently isolated in Australia and Africa. M. avium complex (MAC) and M. tuberculosis may also be isolated from skin lesions. DNA probes may be incorporated to aid with more rapid identification of mycobacterial isolates. This will generate an additional charge(s). Susceptibility testing will be performed only on the first Mycobacterium isolate from each specimen type per patient. Additional susceptibilities may be performed after a period of 4 weeks for patients with suspected treatment failure. Susceptibility testing is performed routinely only for M. tuberculosis. Susceptibility testing for other Mycobacterium species is performed by special request only. The battery of drugs routinely tested will vary by isolate and/or physician request. Patients from whom M. tuberculosis is isolated in culture are reported to the City of Chicago Board of Health. This is mandated by law.

LAST UPDATED:

5-3-2014

TEST SYNONYM(S):AFB Culture, Cutaneous; AFB Culture, Skin; Mycobacterium Culture; TB Culture

Sputum Culture, Fungus

CERNER / EPIC MNEMONIC: C FUNGUSSM
POE DESCRIPTION: CU FUNGUS W SMEAR(NON-SKIN); C FUNGUSSM (NON-SKIN)
CPT CODE: 87102; 87206
TEST INFORMATION: Test includes a culture for fungi and performance of a direct fungal smear. All fungal isolates will be identified except for yeast that have the morphologic appearance of Candida Spp. and that have been shown not  to be Cryptococcus spp.  Such yeast will be identified only as, ''Candida species, not cryptoccus.'' Other isolated organisms (i.e. aerobes, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED:

Daily, 24 hours

TURNAROUND TIME: Smear results: same day if the specimen is received in the laboratory no later than 1400. Preliminary culture reports are available after 1 week or when a fungal isolate is detected. Culture reports will be finalized after 4 weeks.
SPECIAL INSTRUCTIONS: The specific anatomic site of the specimen MUST be specified on the requisition or order.
CONTAINER TYPE: Sterile sputum container; sterile Leuken`s tube; sealed, sterile container, no preservative
COLLECTION: The sputum and Leuken`s specimens should be first morning specimens. Nonbacteriostatic saline should be used when irrigation is necessary. The gastric aspirate is to be collected utilizing aseptic technique. The patient should NOT have eaten within the previous 5 hours when obtaining a gastric aspirate.
SPECIMEN REQUIREMENTS: First morning sputum, induced sputum, aspirated sputum, tracheal aspirate, bronchial, Leuken`s, gastric aspirate
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative.
MINIMUM VOLUME: 2 mL
HANDLING INSTRUCTIONS: Gastric aspirates should be transported immediately. Specimens should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE: No fungi observed/isolated
LIMITATIONS OF TEST: The Calcofluor white stain demonstrates yeast forms, spores, and the hyphae of fungi. The filaments of Nocardia species, Streptomyces species, and Actinomyces species, as well as, the capsule of Cryptococcus species are NOT satisfactorily demonstrated with this methodology.
METHODOLOGY: Fungal smear: calcofluor white stain. Culture: Conventional culture utilizing media specifically formulated for fungal isolation.
ADDITIONAL INFORMATION: Gastric aspirates are frequently diluted with saline. Please notify the Clinical Microbiology Laboratory (312-942-5452) if Histoplasma capsulatum, Blastomyces dermatitidis, or Coccidioides immitis is suspected. These pathogens are highly infectious when propagated in vitro and have been associated with serious infections in exposed laboratory workers.
LAST UPDATED:

5-27-2014

TEST SYNONYM(S):Gastric Aspirate Fungus Culture

Sputum Culture, Mycobacterium

CERNER / EPIC MNEMONIC: C AFBSM
POE DESCRIPTION: CU MYCOBACTERIUM W SMEAR; CU AFB AND SMEAR; C AFBSM
CPT CODE: 87117; 87206
TEST INFORMATION: Test includes culture and acid-fast stain (if specimen volume is adequate). Culture includes decontamination and concentration. If a mycobacterial species is isolated, the organism will be definitely identified. Susceptibility testing will be performed depending upon the isolate identification. Other isolated organisms (i.e. yeast, etc.) may be referred for identification if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily
TURNAROUND TIME: Smear results: 24 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 1 week or when a mycobacterial isolate is detected. Negative cultures with negative smears will be finalized after 8 weeks. Negative cultures with positive smears will be finalized after 12 weeks. Completion of culture reports may require additional time when mycobacteria are isolated and/or susceptibility testing is performed.
CONTAINER TYPE: Sterile sputum container; sterile Leuken`s tube; sealed, sterile container, no preservative
COLLECTION: The sputum and Leuken`s specimens should be first morning specimens. Nonbacteriostatic saline should be used when irrigation is necessary. The gastric aspirate is to be collected utilizing aseptic technique. The patient should NOT have eaten within the previous 5 hours when obtaining a gastric aspirate.
SPECIMEN REQUIREMENTS: First morning sputum, induced sputum, aspirated sputum, tracheal aspirate, bronchial, Leuken`s, gastric aspirate
REJECTION CRITERIA:

Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative. Only one sputum specimen per 8 hours - with one sputum first morning collection. Subsequent specimens will be rejected. Specimens from known positive patients will be accepted only once a week for culture and smear per body site.

MINIMUM VOLUME:

3 mL

HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No acid-fast bacilli observed on smear or isolated in culture
LIMITATIONS OF TEST: A single negative smear does not rule out the presence of mycobacterial infection. Gastric aspirates are frequently diluted with saline.
METHODOLOGY: Acid-fast smear: fluorochrome stain. Culture: inoculation of conventional culture media and a rapid medium for automated continuous analysis.
ADDITIONAL INFORMATION:

The recommended screening procedure is three first morning specimens on three consecutive days or 1 first morning specimen and 2 additional specimens each at least 8 hours apart. DNA probes may be incorporated to aid with more rapid identification of mycobacterial isolates. This will generate an additional charge(s). Susceptibility testing will be performed only on the first Mycobacterium isolate from each specimen type per patient. Additional susceptibilities may be performed after a period of 4 weeks for patients with suspected treatment failure. Susceptibility testing is performed routinely only for M. tuberculosis complex. Susceptibility testing for other Mycobacterium species is performed by special request only. The battery of drugs routinely tested will vary by isolate and/or physician request. Patients from whom M. tuberculosis is isolated in culture are reported to the City of Chicago Board of Health. This is mandated by law.

LAST UPDATED:

5-13-2014

TEST SYNONYM(S):Mycobacterium Culture, Sputum; Sputum, AFB Culture; Sputum, TB Culture; TB Culture, Sputum; AFB Culture, Sputum

Sputum Culture, Routine

CERNER / EPIC MNEMONIC: C SPUTUMGS
CPT CODE: 87070 (culture); 87205 (sceen); 87205 (Gram stain)
CDM NUMBER: 3421053; 3421059; 3421033
TEST INFORMATION:

The test includes a screen for specimen acceptability with few exceptions. The exceptions include: bronchial specimens, specimens obtained from patients less than 6 years of age, and specimens ( swab specimens) from patients reported to the laboratory as having a diagnosis of cystic fibrosis. The test also includes a direct Gram stain, isolation and identification of microorganisms, and susceptibility testing, if appropriate.

DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME:

Gram stain: 2 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 24 hours. Cultures with no growth or normal flora only will be finalized after 3 days. Cultures from patients reported to the laboratory as having a diagnosis of cystic fibrosis will be finalized after 5 days. Complete reports of cultures from which pathogens are isolated may take 3-10 days after receipt of the culture depending upon the nature of the microorganisms detected.

SPECIAL INSTRUCTIONS: The requisition MUST state if the patient has been diagnosed with cystic fibrosis. Patients known to be leukopenic should have this indicated on the requisition.
CONTAINER TYPE: Sterile sputum container; sterile Leuken`s tube; sealed, sterile container, no preservative.
COLLECTION: The sputum and Leuken`s specimens should be first morning specimens. Nonbacteriostatic saline should be used when irrigation is necessary.
SPECIMEN REQUIREMENTS: First morning sputum, induced sputum, aspirated sputum, tracheal aspirate, bronchial, Leuken`s, gastric aspirate
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative, specimen consisting of excessive oropharyngeal contamination as determined by sputum screen, specimen containing food particles. If the specimen is microscopically consistent with saliva, it will be rejected. Only one specimen will be accepted per day.
MINIMUM VOLUME: 2 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: Normal upper respiratory flora
METHODOLOGY: Inoculation of conventional culture media.
ADDITIONAL INFORMATION: The quantity and presence of normal upper respiratory flora will be reported. Normal flora includes alpha and nonhemolytic streptococci, saprophytic Neisseria species, cogulase-negative Staphylococcus, Micrococcus species, and diphtheroids, Haemophilus spp. not influenzae, Candida spp. not cryptococcus. Potential pathogens will be isolated, identified, and susceptibility testing performed, if appropriate. Burkholderia cepacia is identified in the Clinical Microbiology Laboratory. The isolate will be forwarded to the CF Foundation reference laboratory for confirmation and genotyping. The culture report will not be finalized until the reference laboratory confirmation is complete.
LAST UPDATED:

5-27-2014

Sterility Culture

CERNER / EPIC MNEMONIC: C STERILE; C OSTERILE (RML Laboratories ONLY)
POE DESCRIPTION: CU ENVIROMENTAL
CPT CODE: 87071
CDM NUMBER: 3422003
TEST INFORMATION: The test includes isolation and identification of microorganisms. Susceptibility testing is NOT performed. Quantitation of microorganisms may be provided.
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Preliminary culture reports are available after 24 hours. Cultures with no growth will be finalized after 72 hours. Completion of culture reports may take 3-5 days after receipt of the culture when multiple isolates are found and identifications are requested.
SPECIAL INSTRUCTIONS: Specify source of the specimen and whether quantitation and/or identification of microorganisms is required. The following information must be specified: client number, name of a contact person, and the laboratory submitting the specimen.
CONTAINER TYPE: Sealed sterile container, no preservative.
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME: 1 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No growth
METHODOLOGY: Conventional culture media; quantitation may be provided for fluid specimens.
ADDITIONAL INFORMATION: This test should be used to assess the sterility or determine the pathogen-free status of materials or equipment utilized in patient care and/or non-patient care areas. Susceptibility testing is NOT performed. Identification may consist of an isolate Gram stain description or an isolate genus name only based upon the type of specimen submitted. Quantitation may NOT be provided based upon the type of specimen submitted.

Stool Culture, Routine

CERNER / EPIC MNEMONIC: C STOOL
CPT CODE: 87045
CDM NUMBER: 3421023; 3421001; 3451021; 3421081
TEST INFORMATION:

The test includes the isolation and identification of Salmonella species, Shigella species, Aeromonas species, Plesiomonas shigelloides and/or E. coli 0157:H7d; susceptibility testing, if appropriate. The test also includes an assay for the presence of Campylobacter species.  Yersinia species and Vibrio species cultures will be performed ONLY if specifically requested.

DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Campylobacter screening results are available within 1-2 days. Preliminary negative culture reports are available after 48 hours. Culture reports of no enteric pathogens will be issued after 3 days. Isolates presumptively identified as either Salmonella species, Shigella species, or E. coli 0157:H7 must be referred to the Illinois Department of Public Health for confirmation and serogrouping. Isolation of Vibrio species and/or Yersinia species may require an additional 1-2 days for completion.
SPECIAL INSTRUCTIONS:

The request for isolation of Vibrio species and Yersinia species MUST be specified on the requisition.

CONTAINER TYPE: Sealed plastic feces container, no preservative; sealed sterile or nonsterile clean container; Copan Swab II, Para-Pak Carey-Blair transport vial
COLLECTION: The specimen should be collected directly into the plastic feces specimen container (no preservative) or into a bedpan, avoiding contamination with urine or water. Transfer the feces from the bedpan into the plastic feces container or a sealed container.
SPECIMEN REQUIREMENTS: Fresh stool, rectal swab. However, swabs are unacceptable for Campylobacter screening. A culture for Campylobacter spp. will be performed.
REJECTION CRITERIA: Specimens more than 2 hours old, unless preserved in Carey-Blair transport medium, inappropriate specimen container, insufficient specimen volume, specimen contaminated with urine and/or water, more than one specimen submitted to the laboratory per day, specimen submitted more than 3 days after hospitalization, specimen not submitted in a sealed container. Diapers and specimens on tissue paper are NOT acceptable.
MINIMUM VOLUME: 1-2 gram (for stool) or swab showing fecal material
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE:

Negative for Salmonella spp., Shigella spp., Campylobacter spp., Aeromonas spp., Plesiomonas spp. and E. coli 0157.

LIMITATIONS OF TEST:

Specimens will routinely be screened for Salmonella species, Shigella species, Campylobacter species, Aeromonas species, E. coli 0157d, and Plesiomonas species only. A Campylobacter screen CANNOT be performed on a rectal swab.

METHODOLOGY:

Culture: Conventional culture utilizing media specifically formulated for enteric pathogen isolation.Campylobacter screen: Enzyme immunoassay (EIA).

ADDITIONAL INFORMATION:

Organisms other than Salmonella species, Shigella species, and Campylobacter species, Aeromonas spp., Plesiomonas spp. and E. coli 0157 may not be isolated unless specifically indicated on the requisition that other specific isolates are potential etiologic agents, resulting in inoculation of specific media for isolation of alternative pathogens. In enteric fever caused by Salmonella, blood cultures may be positive before stool cultures; therefore, blood cultures are indicated early. Diagnosis of amebic dysentery requires a test request for ova and parasite examination. Patients who develop diarrhea after more than 3 days of hospitalization rarely are infected with enteric pathogens. A test for Clostridium difficile toxin is most appropriate in those cases.

LAST UPDATED:

6-3-2014

TEST SYNONYM(S):Enteric Pathogens Culture, Routine; Rectal Swab for Routine Culture; Stool for Routine Culture

Suprapubic Puncture Anaerobic Culture, Urine

CERNER / EPIC MNEMONIC: C ANAEROBE
CPT CODE: 87075
CDM NUMBER: 3451003
TEST INFORMATION: Test includes culture for anaerobic organisms. Other isolated organisms (i.e. aerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME:

Cultures are examined every 48 hours. Preliminary culture reports are available after 48 hours. Culture reports of no growth will be issued after 5 days. Completion of culture reports may take 7-10 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.

SPECIAL INSTRUCTIONS: Avoid exposure to atmospheric oxygen. Specimens should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. The specific source of the urine specimen MUST be specified on the requisition.
CONTAINER TYPE:

BBL Port-A-Cul Transport System; sealed sterile container

COLLECTION: The specimen of choice is an aspirate, NOT a swab. Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) anaerobes. Ideally, material is obtained by needle aspiration through an intact surface, which has been cleaned with antiseptic, then placed directly into the BBL Port-A-Cul Transport System. When a syringe is used to obtain the specimen, ALL air should be expelled after which the specimen should be injected into a BBL Port-A-Cul Transport System. Contamination with normal flora from skin or other body sources MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
SPECIMEN REQUIREMENTS: Urine obtained by suprapubic puncture
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen. Specimens from sites which have anaerobic bacteria as normal flora will routinely be rejected (i.e. voided. catheterized urine specimens).
MINIMUM VOLUME: 1 mL
HANDLING INSTRUCTIONS: Avoid exposure to atmospheric oxygen. Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate.
REFERENCE RANGE: No growth of anaerobic bacteria
LIMITATIONS OF TEST:

A specimen received in anaerobic transport devices is not suitable for routine fungal or mycobacterial cultures. If more than three anaerobic isolates are present, the Bacteroides fragilis group, and Clostridium perfringens will be the only isolates identified. Anaerobic susceptibility testing is NOT performed routinely. Should susceptibility testing be required, call the laboratory.

METHODOLOGY: Conventional culture utilizing media specifically formulated for anaerobic isolation.
LAST UPDATED:

5-13-2014

TEST SYNONYM(S):Anaerobic Culture, Urine; Suprapubic Puncture; Urine, Suprapubic, Anaerobic Culture

Systemic Fungal Profile, ID

CERNER / EPIC MNEMONIC: SFP
POE DESCRIPTION: SYSTEMIC FUNGAL PROFILE; SFP
CPT CODE: 86606; 86612; 86698; 86635
DAYS PERFORMED: Monday-Saturday
TURNAROUND TIME: 2-4 days
CONTAINER TYPE:

Red top Vacutainer® tube or SST tube

SPECIMEN REQUIREMENTS: Blood (serum)
REJECTION CRITERIA: Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME: 5 mL blood (1 mL serum)
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No Histoplasma antibody detected

No Blastomyces antibody detected

No Coccidioides antibody detected

No Aspergillus antibody detected

METHODOLOGY: Immunodiffusion (ID)
REFERRAL LABORATORY:

Commercial laboratory

TEST SYNONYM(S):SFP; Fungal Immunodiffusion

Skin Biopsy, Immunofluorescence

CPT CODE: 88307; 88346
TEST INFORMATION: Test includes anti-IgG, anti-IgA, anti-IgM, anti-C3, anti-Clq, antifibrinogen immunofluorescence
DAYS PERFORMED: Mon-Fri, 0730-1700
TURNAROUND TIME: 1 week
SPECIAL INSTRUCTIONS: Outside institutions should contact Client Service Representative (312-942-7958) for submission and transport protocol.
CONTAINER TYPE: Weighted polypropylene vial, isopentane, liquid nitrogen filled thermos, obtain from Laboratory Receiving Station, Rm 508 Jelke.
COLLECTION: Take biopsies from the following sites: If pemphigus or bullous pemphigoid is suspected and fresh lesions are present, take a 3 mm biopsy at the edge of the bulla. If only old lesions are available, biopsy adjacent area. If dermatitis herpetiformis is suspected, biopsy lesion and uninvolved area around lesions. If systemic lupus erythematosus is suspected, biopsy sun-exposed normal skin, preferably the wrist or deltoid area.
SPECIMEN REQUIREMENTS: 3 mm skin punch biopsy or mucous membrane biopsy
REJECTION CRITERIA: Formalin-fixed tissue, improper or discrepant labeling.
HANDLING INSTRUCTIONS: Specimen submitted fresh or in saline-moistened gauze should be placed into the vial with isopentane after the isopentane has been precooled in liquid nitrogen. The vial with the specimens should then be immersed in the liquid nitrogen and transported immediately.

Special Stain Profile

CERNER / EPIC MNEMONIC: SPEC STAIN
CPT CODE: 88319; 88313 (x4)
TEST INFORMATION: Test includes Alpha-Naphthyl-Acetate Esterase Stain With and Without Fluoride; Naphthol ASD Chloroacetate Esterase Stain; PAS Stain; Peroxidase Stain; Sudan Black B Stain
DAYS PERFORMED: Mon-Fri ,0800-1600
TURNAROUND TIME: 24-48 hours
SPECIAL INSTRUCTIONS: Test performed automatically on newly diagnosed cases of acute leukemia. Call 942-5260 to speak to a hematopathologist for all other cases.
CONTAINER TYPE: Lavender top (EDTA) tube
COLLECTION: Specimen obtained by Bone Marrow Laboratory technologist.
SPECIMEN REQUIREMENTS: Blood or bone marrow smears prepared by laboratory technologists; imprints or smears of cell suspension
REJECTION CRITERIA: Air-dried slides >7 days old.
MINIMUM VOLUME: 7 mL
HANDLING INSTRUCTIONS: Deliver to the laboratory immediately.
REFERENCE RANGE: Interpreted by the hematopathologist. Phone Surgical Pathology, 942-5260 for results.
TEST SYNONYM(S):Cytochemistry, Leukocyte; Leukocyte Cytochemistry

STR Chimerism/Engraftment Analysis by PCR

CERNER / EPIC MNEMONIC: ENG
POE DESCRIPTION: CHIMERISM, BONE MARROW ENGRAFTMENT
CPT CODE:

83891, 83898, 83909, 83912, 83903

With T Cell Extraction (ENG T) - 83891 x2, 83898 x2, 83909 x2, 83903, 83912

DAYS PERFORMED: Mon - Thurs, 0900-1600; Fri, 0900-1200
TURNAROUND TIME: 7-10 days
SPECIAL INSTRUCTIONS: Must be accompanied by patient information sheet. STRI results for donor and pre-transplant recipient are required.
CONTAINER TYPE: Yellow top (ACD) or lavender top (EDTA) tube
SPECIMEN REQUIREMENTS: Bone marrow/ 2-3 ml, blood/ 5-10 ml, depending on WBC count
MINIMUM VOLUME: Bone marrow/ 1-2 ml, blood/ 2-3 ml, if WBC count 3,000/microliter
HANDLING INSTRUCTIONS: Store tubes at room temperature; deliver to the Cellular Laboratory (1188 Jelke) or the Molecular Laboratory (240B Rawson) or tube to Microbiology #309 within 24 hours.
REFERENCE RANGE: less than 1 - more than 99% engraftment 

STR Informative Analysis by PCR

CERNER / EPIC MNEMONIC: STRI
CPT CODE: 83891, 83900, 83909, 83903, 83912
DAYS PERFORMED: Mon - Thurs, 0900-1600; Fri, 0900-1200
TURNAROUND TIME: 7-10 days
SPECIAL INSTRUCTIONS: Donor and pre-transplant recipient specimens must be accompanied by patient information sheet.
CONTAINER TYPE: Yellow top (ACD) or lavender top (EDTA) tube
SPECIMEN REQUIREMENTS: Bone marrow/ 2-3 ml, blood/ 3-5 ml, depending on WBC count, 6 buccal swabs
MINIMUM VOLUME: Bone marrow/ 1-2 ml, blood/2-3 ml, 2 buccal swabs
HANDLING INSTRUCTIONS: Store tubes at room temperature; deliver to the Cellular Laboratory (1188 Jelke) or the Molecular Laboratory (240B Rawson) or tube to Microbiology #309 within 24 hours.
REFERENCE RANGE: D5S818, D7S820, D13S317, D16S539, TH01, vWA, F13A01, FESFPS, F13B, TPOX, CSF1PO, LPL, and amelogenin loci screened

Serum Viscosity

CERNER / EPIC MNEMONIC: SER VIS
CPT CODE:

85810-90

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE: Red top tube - 10 mL
SPECIMEN REQUIREMENTS:

4 mL blood (2 mL serum)

REJECTION CRITERIA:

Plasma

MINIMUM VOLUME:

2 mL blood, (1 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 1240

LIMITATIONS OF TEST:

Marked lipemia or marked hemolysis may cause falsely increased results.

METHODOLOGY:

Viscometry

TEST SYNONYM(S):Viscosity, serum

Serotonin, Urine

CERNER / EPIC MNEMONIC: U/5-HIAA
POE DESCRIPTION: URT HYDROXYINDOLEACETIC ACID; URT 5HIAA
CPT CODE:

83497-90

TURNAROUND TIME:

5-7 working days

SPECIAL INSTRUCTIONS:

Patient should abstain, if possible, from medications, over-the-counter drugs and herbal remedies for at least 72 hours prior to collection.  Patients should not eat avocados, bananas, eggplant, pineapple, plums, tomatoes or walnuts for a 48-hour period prior to start of urine collection.

CONTAINER TYPE:

24-hour urine bottle, Chemistry bottle #4 (containing 10 mL 6N HCl).  Caution: 6N HC1 is caustic.

or 24-hour urine bottle #1, if kept refrigerated.

SPECIMEN REQUIREMENTS: 24-hour urine
MINIMUM VOLUME: Submit entire urine collection to the laboratory.
HANDLING INSTRUCTIONS: Refrigerate during collection
REFERENCE RANGE: See Specialty Labs - Search Test Code 3310U
TEST SYNONYM(S):5-Hydroxyindoleacetic Acid, Quantitative, Urine; 5-HIAA, Quantitative, Urine; Serotonin Metabolite; Urine 5-HIAA

Scleroderma Antibody

CERNER / EPIC MNEMONIC: SCLERODERM
POE DESCRIPTION: SCLERODERMA ANTIBODY; SCLERODERM
CPT CODE: 86235-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Red top tube or SST tube

SPECIMEN REQUIREMENTS: 2.0 mL blood (1.0 mL serum)
MINIMUM VOLUME:

1.0 mL blood (0.5 mL serum)

REFERENCE RANGE: See Specialty Labs - Search Test Code 1235

TEST SYNONYM(S):Progressive Systemic Sclerosis Antibody; SCL-70

Serotonin, Blood

CERNER / EPIC MNEMONIC: SEROTONIN
POE DESCRIPTION: SEROTONIN
CPT CODE: 84260-90
TURNAROUND TIME: 5-7 working days
SPECIAL INSTRUCTIONS: Patient should avoid food avocados, bananas, eggplant, pineapples, plums, tomatoes, or walnuts, for a 48-hour period prior to start of collection. This test cannot be added onto an existing sample.
CONTAINER TYPE: Red top tube only
SPECIMEN REQUIREMENTS: 2.0 mL whole blood (1.0 mL serum)
MINIMUM VOLUME: 1.0 mL whole blood (0.5 mL serum)
REFERENCE RANGE: See Specialty Labs - Search Test Code 3286

TEST SYNONYM(S):5-Hydroxytryptamine, Blood

Sex Hormone Binding Globulin

CERNER / EPIC MNEMONIC: SHBG
POE DESCRIPTION: SHBG
CPT CODE: 84270-90
TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

Red top tube or SST tube

SPECIMEN REQUIREMENTS: 2.0┐mL blood (1.0 mL serum)
MINIMUM VOLUME: 1.0 mL blood (0.4 mL serum)
REFERENCE RANGE: See Specialty Labs  - Search Test Code 3218

TEST SYNONYM(S):SHBG; Testosterone Binding Globulin

Smooth Muscle Antibodies

CERNER / EPIC MNEMONIC: ASMA
POE DESCRIPTION: SMOOTH MUSCLE AB; ASMA
CPT CODE:

86255-90

TURNAROUND TIME: 5-7 working days
CONTAINER TYPE: Red top tube or SST(TM) tube
SPECIMEN REQUIREMENTS: 2.0 mL blood (1 mL serum)
MINIMUM VOLUME: 1.0 mL blood (0.5 mL serum)
REFERENCE RANGE: See Specialty Labs  - Search Test Code 1106

TEST SYNONYM(S):Antismooth Muscle Antibody; ASMA

Sodium, Feces: 24, 48 or 72 hrs

CERNER / EPIC MNEMONIC: Q/NA
POE DESCRIPTION: FEC NA; FEC SODIUM; FEC SODIUM (RANDOM SPECIMEN); Q/NA; RQ/SODIUM
CPT CODE: 84302-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE: One or more stool containers from Core Laboratory
SPECIMEN REQUIREMENTS: 24, 48, or 72-hour stool collection. Submit entire collection to the laboratory. Specimen must be liquid.
MINIMUM VOLUME: Entire collection
HANDLING INSTRUCTIONS: Refrigerate during collection.
REFERENCE RANGE:

See Quest Diagnostics Lab - Search Test Code - 8833

 

TEST SYNONYM(S):Sodium, Stool; Fecal Sodium

Sezary Cell Smear

CERNER / EPIC MNEMONIC:

Sezary SM

POE DESCRIPTION:

Sezary Cell Smear

TEST INFORMATION:

A physician, usually a Dermatologist, may ask that a smear be reviewed for sezary cells, associated with the disease, mycosis fungoides (a peripheral T-cell lymphoma).

DAYS PERFORMED:

Monday-Friday

TURNAROUND TIME:

48 hours

CONTAINER TYPE:

Lavender top

COLLECTION:

VENI

MINIMUM VOLUME:

1.5 mL (EDTA Whole Blood)

METHODOLOGY:

Microscopy

Selenium, blood

CERNER / EPIC MNEMONIC:

SELENIUM

POE DESCRIPTION:

Selenium

CPT CODE:

84255-90

CDM NUMBER:

3181391

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

Dark blue top (EDTA) tube (Vacutainer #369736) or Dark blue top (heparin) tube (Vacutainer #369735)

SPECIMEN REQUIREMENTS:

2.0 mL EDTA whole blood

MINIMUM VOLUME:

1.0 mL EDTA whole blood

REFERENCE RANGE:

See Specialty Labs  - Search Test Code 4875W

METHODOLOGY:

Atomic absorption

TEST SYNONYM(S):SE

Sirolimus

CERNER / EPIC MNEMONIC: SIROLIMUS
POE DESCRIPTION: SIROLIMUS; RAPAMYCIN (SIROLIMUS);

RAPAMUNE (SIROLIMUS)

CPT CODE: 80299
DAYS PERFORMED:

Test is performed Monday-Sunday.

TURNAROUND TIME:

Up to 18 hours.

SPECIAL INSTRUCTIONS:

Draw time must be indicated on requisition.

CONTAINER TYPE: Lavender top (K2 EDTA) tube - 6 mLLavender top (K2 EDTA) tube - 3 mLMicrotainer (K2 EDTA)Pink top (K2 EDTA) tube - 6 mL
SPECIMEN REQUIREMENTS:

1.0 mL EDTA whole blood

MINIMUM VOLUME:

0.5 mL EDTA whole blood

REFERENCE RANGE:

The effective therapeutic range for Sirolimus is based upon clinical evaluation of the individual patient, because optimal levels depend upon multiple factors, including patient variation, type of transplant, time post-transplant, co-administration of other immunosuppressants, and method used.

TEST SYNONYM(S):Rapamune; Rapamycin

Salmon (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

SALMON

POE DESCRIPTION:

Salmon allergen

CPT CODE:

86003-90

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code F41

Scallop (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

SCALLOP

POE DESCRIPTION:

Scallop allergen

CPT CODE:

86003-90

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code RF338

Shrimp (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

SHRIMP

POE DESCRIPTION:

Shrimp allergen

CPT CODE:

86003-90

TEST INFORMATION:

Included in the Rast Food Panel.

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code F24

Soybean (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

SOYBEAN

POE DESCRIPTION:

Soybean allergen

CPT CODE:

86003-90

TEST INFORMATION:

Included in the Rast Food Panel.

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code F14

Sweet vernal grass (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

SWT. VERNAL

POE DESCRIPTION:

Sweet vernal grass allergen

CPT CODE:

86003-90

TEST INFORMATION:

Included in the Rast Inhalant Panel.

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs  - Search Test Code G1

Saccharomyces Cerevisiae IgG & IgA Antibodies

CERNER / EPIC MNEMONIC:

SAC CEREAB

CPT CODE:

86671-90 (x2)

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube

SPECIMEN REQUIREMENTS:

2 mL blood (1 mL serum)

MINIMUM VOLUME:

1 mL blood (0.5 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 1445

METHODOLOGY:

EIA

Striated Muscle Antibody

CERNER / EPIC MNEMONIC:

STRIAT AB

POE DESCRIPTION:

Striated Muscle Antibodies

CPT CODE:

86255-90

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST TubeRed top tube - 10 mLRed top tube - 4 mLMicrotainer (SST),   Microtainer (Red)

SPECIMEN REQUIREMENTS:

2 ml blood (1 mL serum)

MINIMUM VOLUME:

1 mL blood (0.5 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 1107

METHODOLOGY:

IFA

Strep Pneumo 23 Valent Panel

CERNER / EPIC MNEMONIC:

STREP PN23

CPT CODE:

86317 X23

TEST INFORMATION:

Test includes antibody levels for Streptococcus pneumoniae types 1,2,3,4,5,8,9(9N),12(12F),17(17F),14,19(19F),20,22(22F),23 (23F),26(6B) 34(10A),43(11A),51(7F),54(15B),56(18C),57(19A),68(9V),70(33F)

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or Red Top tube

SPECIMEN REQUIREMENTS:

1.5 mL blood (0.5 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.25 mL serum)

REFERENCE RANGE:

See Specialty Laboratory - Search Test Code 16963

LAST UPDATED:

3-11-2014

TEST SYNONYM(S):Strep Pneumo Antibody Screen

Strawberry IgE Rast Allergen

CERNER / EPIC MNEMONIC:

SBERRY

CPT CODE:

86003-9

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.5 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code F44

LAST UPDATED:

3/06/2013

Strongyloides IgG Antibody, ELISA

CERNER / EPIC MNEMONIC:

STRONG AB

POE DESCRIPTION:

STRONGYLOIDES IGG AB

CPT CODE:

86682

TEST INFORMATION:

Strongyloides stercoralis is a parasitic nematode found in tropical and subtropical regions. Because of low larval densities in feces, stool examination is a relatively insensitive diagnostic test; serodiagnosis by ELISA offers increased sensitivity. Antibody titers decrease in many patients following treatment. Patients with latent infections who are immunosuppressed or receiving immunosuppressive therapy are at risk life-threatening hyperinfection. The assay shows 90% sensitivity and 90% specificity.

DAYS PERFORMED:

Monday, Wednesday, Friday

TURNAROUND TIME:

1 - 4 Days

CONTAINER TYPE:

Red Top

COLLECTION:

1 mL Serum

REJECTION CRITERIA:

Insufficient quantity of specimen, inappropriate specimen collection tube

MINIMUM VOLUME:

0.5 mL

HANDLING INSTRUCTIONS:

Specimen stability: Room temperature 7 days, Refrigerated 14 days, Frozen 30 days

REFERENCE RANGE:

See Quest Test Code 34309

LIMITATIONS OF TEST:

Significant crossreactivity may be observed with filarial and other nematode infections.

METHODOLOGY:

Immunoassay

REFERRAL LABORATORY:

QUEST DIAGNOSTICS

LAST UPDATED:

6-25-2013

TEST SYNONYM(S):Strongyloides serology

Semen Analysis Without Morphology

CERNER / EPIC MNEMONIC:

SEMEN ONLY

POE DESCRIPTION:

SEMEN ANALYSIS W/O MORPHOLOGY (Count and Motility only)

CPT CODE:

89321

DAYS PERFORMED:

Monday-Friday, 0700 am - 1100 am with appointment

SPECIAL INSTRUCTIONS:

Call office to schedule appointments.  Appointment should be scheduled at least 3 days in advance.  You must receive a Medical Record Number in Room 104 Pro Bldg I prior to producing semen specimen.  Semen specimens are produced in the Andrololgy Lab, Suite 119 Pro Bldg I.  Please allow 2-3 days of sexual abstinence prior to specimen collection.

CONTAINER TYPE:

Sterile specimen container (provided by lab)

SPECIMEN REQUIREMENTS:

Semen

REJECTION CRITERIA:

Improper collection, incomplete requisition information

MINIMUM VOLUME:

1.0

HANDLING INSTRUCTIONS:

Specimen should ideally be produced in lab

REFERENCE RANGE:

On form

LAST UPDATED:

9-5-2013

Semen Cryopreservation

CERNER / EPIC MNEMONIC:

CRYOPRES

POE DESCRIPTION:

SEMEN CRYOPRESERVATION

CPT CODE:

89259

DAYS PERFORMED:

Monday-Friday, 0700 am - 1100 am with appointment

TURNAROUND TIME:

3-5 business days

SPECIAL INSTRUCTIONS:

Call office to schedule appointments.  Appointment should be scheduled at least 3 days in advance.  You must receive a Medical Record Number in Room 104 Pro Bldg I prior to producing semen specimen.  Semen specimens are produced in the Andrololgy Lab, Suite 119 Pro Bldg I.  Please allow 2-3 days of sexual abstinence prior to specimen collection.

CONTAINER TYPE:

Sterile specimen container (provided by lab)

SPECIMEN REQUIREMENTS:

Semen

REJECTION CRITERIA:

Improper collection, incomplete requisition information

MINIMUM VOLUME:

1.0

HANDLING INSTRUCTIONS:

Specimen should ideally be produced in lab.

REFERENCE RANGE:

On form

LAST UPDATED:

9-5-2013