Magnesium, Quantitative, Urine

CERNER / EPIC MNEMONIC: RU/MG (random urine); U/MG (24-hour urine)
POE DESCRIPTION:

Urine Magnesium Random; Urine Magnesium Timed

CPT CODE: Random = 83735, Timed =  83735, 81050
DAYS PERFORMED: Specimen accepted daily, 24 hours
TURNAROUND TIME: 8 hours
SPECIAL INSTRUCTIONS: For timed urine collection, instruct the patient to void at the beginning of the collection period and discard the specimen. Then collect all urine including the final specimen voided at the end of the collection period. Containers must be labeled with patient`s full name, room number, date and time collection started, and date and time collection finished.
CONTAINER TYPE:

Random Sterile 80 ml specimen container, Timed 24 hour urine container (Bottle #1) with no preservative or 24 hour urine container (Bottle #4) with 10 ml 6 N HCl

SPECIMEN REQUIREMENTS: Random or 24-hour urine
MINIMUM VOLUME: Random urine: 1 mL; Timed urine: Submit entire collection to the laboratory.
HANDLING INSTRUCTIONS: Refrigerate during collection.
REFERENCE RANGE:

Random: None established; Timed urine: 75-150 mg/24 hours

LAST UPDATED:

3-22-2013

TEST SYNONYM(S):Mg, Urine; Urine Magnesium

Magnesium, Serum

CERNER / EPIC MNEMONIC: MG
POE DESCRIPTION: MAGNESIUM; MG; MG
CPT CODE: 83735; 83735
DAYS PERFORMED: Specimen accepted daily, 24 hours
TURNAROUND TIME: Routine: 8 hours; stat: 1 hour
CONTAINER TYPE:

SST tube

SPECIMEN REQUIREMENTS: Blood (serum)
MINIMUM VOLUME: 0.4 mL blood (0.2 mL serum)
REFERENCE RANGE: 1.6-2.7 mg/dL
TEST SYNONYM(S):Mg

Malaria Smear

CERNER / EPIC MNEMONIC: MALARIA
POE DESCRIPTION: MALARIA; SM MALARIA
CPT CODE: 87207
TEST INFORMATION: Test includes: thick and thin smears
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: 8 hours
SPECIAL INSTRUCTIONS: If the patient has traveled to a malaria endemic area the date and area traveled should be specified on the requistion.
CONTAINER TYPE: Lavender top (EDTA) tube
SPECIMEN REQUIREMENTS: Blood
MINIMUM VOLUME: 1.5 mL whole blood
HANDLING INSTRUCTIONS: Specimen should be sent to the laboratory as soon as possible but not longer than 2 hours after collection.
REFERENCE RANGE: Normal: no organisms identified
TEST SYNONYM(S):Blood Smear for Malarial Parasites; Malarial Parasites

Methemoglobin, Blood

CERNER / EPIC MNEMONIC: METH/HB
POE DESCRIPTION: METHHEMOGLOBIN
CPT CODE: 83050
DAYS PERFORMED: Specimen accepted daily, 24 hours
TURNAROUND TIME: 15 minutes
SPECIAL INSTRUCTIONS: Deliver specimen immediately to the lab on ice.
CONTAINER TYPE:

Heparinized syringe or green top tube (heparin)

SPECIMEN REQUIREMENTS: Heparinized whole blood
REJECTION CRITERIA: Clotted syringe, needle attached to the syringe, and air bubbles in the sample.
MINIMUM VOLUME: 0.3 mL whole blood
HANDLING INSTRUCTIONS: Keep on ice.
REFERENCE RANGE: 0.4% to 1.5%
TEST SYNONYM(S):Ferric-Hemoglobin; Met Hgb; Oxidized Hemoglobin

Methotrexate

CERNER / EPIC MNEMONIC: MTRX
POE DESCRIPTION: METHOTREXATE
DAYS PERFORMED:

Daily - 24 hours

TURNAROUND TIME:

1 hour

SPECIAL INSTRUCTIONS: Draw time must be recorded on requisition.
CONTAINER TYPE: Red top tube - 10 mL or Red top tube - 4 mL
SPECIMEN REQUIREMENTS:

10 mL blood (5.0 mL serum)

MINIMUM VOLUME: 2 mL blood (1.0 mL serum)
REFERENCE RANGE: Questions regarding methotrexate dosing should be referred to Pharmacy. All methotrexate results are called to the requesting physician or nursing unit.
METHODOLOGY:

Fluorescent polarization Immunoassay (FPIA)

Microalbumin, Quantitative, Urine

CERNER / EPIC MNEMONIC: RU/MICROAL (Random Urine); U/MICROALB (24 hour urine)
POE DESCRIPTION:

Urine Microalbumin Random; Urine Microalbumin Timed

CPT CODE: Random = 82043, 82570; Timed = 82043, 81050
TEST INFORMATION:

Random urine microalbumin includes a random urine creatinine and a microalbumin/creatinine ratio.

DAYS PERFORMED:

Specimen accepted daily, 24 hours

TURNAROUND TIME:

8 hours

SPECIAL INSTRUCTIONS: For timed urine collection, instruct the patient to void at the beginning of the collection period and discard the specimen. Then collect all urine including the final specimen voided at the end of the collectionn period. Containers must be labeled with patient`s full name, room number, date and time collection started, and date and time collection finished.
CONTAINER TYPE:

Random Sterile 80 ml specimen container, Timed 24 hour urine container (Bottle #1) with no preservative

SPECIMEN REQUIREMENTS:

Random urine: 1 mL; Timed urine: Submit entire urine collection to the laboratory.

MINIMUM VOLUME: Submit entire urine collection to the laboratory.
HANDLING INSTRUCTIONS: Refrigerate during collection.
REFERENCE RANGE: Random urine: No reference range for random urine microalbumin and creatinine. Reference range for microalbumin/creatinine ratio 0-25 mcg/mg.  Timed urine: 0-25 mg/24 hours
TEST SYNONYM(S):Urine Albumin, Microalb; Urine Microalbumin

Mixing Studies - PT Inhibitor

CERNER / EPIC MNEMONIC: PT INHIB
POE DESCRIPTION: QUICK DILUTION, PT INHIBITOR, MIXING STUDIES
CPT CODE: 85610
DAYS PERFORMED:

Daily, second shift

TURNAROUND TIME: 24-72 hours
SPECIAL INSTRUCTIONS:

This test cannot be added onto an existing sample more than 12 hours old.

CONTAINER TYPE:

Two Blue citrate tubes

COLLECTION: See Section - ''Specimen Collection Guidelines'' - Coagulation Specimens
SPECIMEN REQUIREMENTS:

Two 2.7 mL draw light blue tops

REJECTION CRITERIA: Specimen hemolyzed; Hct >55%; underfilled tube (QNS); clotted specimen; normal protime result
MINIMUM VOLUME:

Two 2.7 mL draw blue tops (2 mL plasma)

HANDLING INSTRUCTIONS: Transport the specimen to the laboratory as soon as possible. Specimen MUST be received within 4 hours after collection.
REFERENCE RANGE: Normal: negative.
LAST UPDATED:

2-27-2014

TEST SYNONYM(S):PT Inhibitor Mixing Study; Extrinsic Inhibitor; Mixing Studies - PT; Prothrombin Time Mixing Studies

MHA-TP, Serum

CERNER / EPIC MNEMONIC:

MHA-TP

CPT CODE: 86781
DAYS PERFORMED:

Monday, first shift

TURNAROUND TIME:

1 week

CONTAINER TYPE:

Red top Vacutainer® tube or SST tube

SPECIMEN REQUIREMENTS: Blood (serum)
REJECTION CRITERIA: Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME: 1 mL blood (0.2 mL serum)
REFERENCE RANGE: Nonreactive
LIMITATIONS OF TEST: The assay may fail to detect antibodies in approximately 30% of patients with early primary syphilis and in 5% of sera from patients with late syphilis.
METHODOLOGY:

Gelatin particle agglutination

ADDITIONAL INFORMATION: The assay, which confirms the presence of antibodies to the pathogenic strain of Treponema pallidum, is used to diagnose syphilis. It is NOT recommended as a screening test for syphilis. The assay should NOT be used to follow treatment or establish reinfection since treponemal antibody tests can remain reactive for the life of treated patients. Patients from whom reactive results are obtained are reported to the City of Chicago Board of Health. This is mandated by law.
TEST SYNONYM(S):HATTS.; TPHA; Treponemal Antibody Test; MHA-TP

Measles (Rubeola) Virus IgG Antibody Titer

CERNER / EPIC MNEMONIC: MEAS IGG
POE DESCRIPTION: MEASLES VIRUS IGG AB TITER; MEAS IGG
CPT CODE: 86765-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

SST tube or Red top Vacutainer® tube

SPECIMEN REQUIREMENTS:

2 mL blood (1 mL serum)

REJECTION CRITERIA: Gross hemolysis, microbial contamination of specimen, hyperlipemia.
MINIMUM VOLUME: 1 mL blood (0.1 mL serum)
REFERENCE RANGE: See Quest Diagnostics - Search Test Code 964
TEST SYNONYM(S):Rubeola Titer

Measles (Rubeola) Virus IgM Antibody

CERNER / EPIC MNEMONIC:

MISC CHEM

POE DESCRIPTION: MEASLES VIRUS IGM AB TITER; MEAS IGM
CPT CODE: 86765-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Red top Vacutainer® tube or SST tube

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

REJECTION CRITERIA: Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME:

1.0 mL blood (0.5 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 34256

METHODOLOGY:

Enzyme immunoassay (EIA)

LAST UPDATED:

9-8-2014

TEST SYNONYM(S):Rubeola IgM Titer

Minimum Bactericidal Concentration

CERNER / EPIC MNEMONIC: MIC/MBC
CPT CODE: 87186; 87187 (each drug)
CDM NUMBER: 3421044; 3421042 (each drug)
DAYS PERFORMED: Monday-Thursday, 0700-1400
TURNAROUND TIME: 2-5 days
SPECIAL INSTRUCTIONS: The patient`s organism must be saved at the request of the physician. The request must be received by the laboratory within 7 days of specimen submission for initial culture in order to perform a MBC test. If the isolate has not been saved, the test cannot be performed. The physician must specify specific bacterial isolate and antimicrobial agent(s) to be tested. The types of antibiotics tested depends upon the organism identification.
SPECIMEN REQUIREMENTS: Organism isolated from patient`s specimen (pure culture isolate)
REJECTION CRITERIA: Organism discarded prior to physician request for testing, non-viable isolate.
REFERENCE RANGE: Variable depending upon the organism and the antibiotic.
METHODOLOGY: Microtiter MIC; macrotube dilution with culture for MBC
REFERRAL LABORATORY: Commercial Laboratory
ADDITIONAL INFORMATION: Antimicrobials not yet FDA approved may be tested on a research use only basis.
TEST SYNONYM(S):MBC

Monotest

CERNER / EPIC MNEMONIC: MONOTEST
POE DESCRIPTION: MONO TEST
DAYS PERFORMED:

Routine:  Performed once daily on first and second shifts.

STATS:  Daily - 24 hours

TURNAROUND TIME:

Routine: 1 day

STATS: 1 hour after receipt of the specimen in the laboratory

CONTAINER TYPE:

Red top tube - 4 mL or SST tube

SPECIMEN REQUIREMENTS:

4 mL blood (2 mL serum)

REJECTION CRITERIA: Any hemolysis, chylous serum.
MINIMUM VOLUME: 2 mL blood (1 mL serum)
REFERENCE RANGE: Negative
LIMITATIONS OF TEST: Correlation with clinical findings is imperative since false positive and negative results have been reported. Approximately 10% of the adult population with infectious mononucleosis will not develop heterophile antibodies. Failure to develop heterophile antibodies occurs even more frequently in children. (Refer to Epstein-Barr Viral Capsid Antigen Antibody Titer.) Less than 2% false positives have been reported with Hodgkin`s disease, lymphoma, acute lymphocytic leukemia, infectious hepatitis, pancreatic carcinoma, Cytomegalovirus, Burkitt`s lymphoma, acute lymphocytic leukemia, and rheumatoid arthritis. The simultaneous occurrence of infectious mononucleosis and hepatitis has been reported.
METHODOLOGY:

Rapid latex particle agglutination.

ADDITIONAL INFORMATION: The test detects heterophile antibodies related to infectious mononucleosis.
TEST SYNONYM(S):Heterophile Agglutinins; Monospot

Mumps IgG Antibody Titer

CERNER / EPIC MNEMONIC: MUMPS IGG
POE DESCRIPTION: MUMPS IGG ANTIBODY TITER; MUMPS IGG
CPT CODE: 86735-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

SST tube or Red top Vacutainer® tube

SPECIMEN REQUIREMENTS:

2 mL blood (1 mL serum)

REJECTION CRITERIA: Gross hemolysis, hyperlipemia
MINIMUM VOLUME: 1 mL blood (0.1 mL serum)
REFERENCE RANGE: See Quest Diagnostics - Search Test Code 8624
TEST SYNONYM(S):Mumps Titer

Mumps IgM Antibody

CERNER / EPIC MNEMONIC:

MISC CHEM

POE DESCRIPTION: MUMPS IGM ANTIBODY TITER; MUMPS IGM
CPT CODE: 86735-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Red top Vacutainer® tube or SST tube

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

REJECTION CRITERIA: Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME:

1.0 mL blood (0.5 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 9721

METHODOLOGY:

Enzyme immunoassay (EIA)

ADDITIONAL INFORMATION: The test is used to aid in the diagnosis of acute Mumps virus infection.
TEST SYNONYM(S):Parotitis Epidemica Antibodies

Mycoplasma pneumoniae Antibody Titer (Total)

CERNER / EPIC MNEMONIC: MPNEUMO AB
POE DESCRIPTION:

MPNEUMO AB TITER MICRO REF

CPT CODE:

86738 x2

DAYS PERFORMED: Monday-Friday
TURNAROUND TIME:

1-3 days

SPECIAL INSTRUCTIONS: Specimen must be received by the laboratory no later than 0800 on the day the test is to be performed. It is the responsibility of the ordering physician to see that both acute and convalescent specimens are obtained. The physician should arrange for the collection of the convalescent serum 10-14 days after the acute is collected.
CONTAINER TYPE:

Red top Vacutainer® tube or SST tube

SPECIMEN REQUIREMENTS: Blood (serum)
REJECTION CRITERIA: Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME: 5 mL blood (1 mL serum)
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE:

IgG <0.91 U/mL negative

IgM <770 U/mL negative

METHODOLOGY:

EIA

REFERRAL LABORATORY:

Commercial

ADDITIONAL INFORMATION: The test is used to aid in the diagnosis of Mycoplasma pneumoniae infection, primarily atypical pneumonia.
LAST UPDATED:

5-27-2014

TEST SYNONYM(S):M. pneumoniae Titer; Mycoplasma Complement Fixation

Mycoplasma pneumoniae Culture (Respiratory)

CERNER / EPIC MNEMONIC: C MYCOPLAS
POE DESCRIPTION:

CU MYCOPLASMA; C MYCOPLAS MICRO REF

CPT CODE: 87109
DAYS PERFORMED: Monday-Friday
TURNAROUND TIME:

2-3 weeks

CONTAINER TYPE: NON-wooden swab placed in Mycoplasma transport medium; sealed sterile container
SPECIMEN REQUIREMENTS: Throat swabs, gargles, sputum, bronchial washings, tracheal aspiration
REJECTION CRITERIA: Inappropriate specimen container, inappropriate transport medium, improper collection of specimen, specimen heavily contaminated with bacteria, wooden swab used in obtaining specimen.
MINIMUM VOLUME:

1 mL or one swab in M4

HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No Mycoplasma isolated
LIMITATIONS OF TEST: Culture may be negative even with Mycoplasma infection
METHODOLOGY: Broth and agar cultures with media specific for isolation of Mycoplasma.
REFERRAL LABORATORY:

Commercial laboratory

LAST UPDATED:

5-27-2014

TEST SYNONYM(S):M. pneumoniae Culture

Mycoplasma/Ureaplasma urealyticum Culture (Genital)

CERNER / EPIC MNEMONIC:

MICRO REF

POE DESCRIPTION:

MICRO REF

CPT CODE: 87109
DAYS PERFORMED:

Monday-Friday

TURNAROUND TIME: 10 days
CONTAINER TYPE:

Sealed sterile container

SPECIMEN REQUIREMENTS: Urethral swab, cervical swab, endocervical swab, expressed prostatic fluid, semen, endometrial tissue, urine.
REJECTION CRITERIA: Inappropriate specimen container, inappropriate transport medium, improper collection of specimen, specimen heavily contaminated with bacteria, wooden swab used in obtaining specimen.
MINIMUM VOLUME:

10-20 mL, 1 swab in M4 TM

HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE: No Mycoplasma isolated

No Ureaplasma urealyticum isolated

LIMITATIONS OF TEST: The culture may be negative in the presence of Mycoplasma T-strain infection. The presence of Mycoplasma does NOT always indicate infection.
METHODOLOGY: Broth and agar cultures with media specific for isolation of Mycoplasma and/or Ureaplasma.
REFERRAL LABORATORY:

Commercial laboratory

ADDITIONAL INFORMATION: The test is used to support the diagnosis of Mycoplasma or Ureaplasma urealyticum infection in suspected cases of urethritis and cervicitis.
LAST UPDATED:

5-27-2014

TEST SYNONYM(S):Genital Culture for Ureaplasma urealyticum; Mycoplasma hominis Culture, Genital; Ureaplasma urealyticum Culture, Genital

Mitogen Stimulation of Lymphocytes: Specify Phytohemagglutinin (PHA), Concanavalin A (Con A) or Pokeweed Mitogen (PWM)

CERNER / EPIC MNEMONIC:

MITOGEN

POE DESCRIPTION: PHYTOHEMAGLUTININ STIMULATION; PHA

CONCAVALIN A; CON A

POKEWEED MITOGEN; PWM

CPT CODE: 86353 for each
DAYS PERFORMED: Mon-Thurs.
TURNAROUND TIME: 2 weeks
SPECIAL INSTRUCTIONS:

Blood must be in laboratory by 1300 and within 3 hours of drawing. Test requires viable lymphocytes. Specimens accepted Monday through Thursday Only.

CONTAINER TYPE: Green top (sodium heparin) tube
SPECIMEN REQUIREMENTS:

10 mL heparinized whole blood

REJECTION CRITERIA:

Lithium Heparin tubes are not acceptable.

MINIMUM VOLUME:

1 mL heparinized whole blood

HANDLING INSTRUCTIONS:

Do not refrigerate.  Specimen must arrive at Mayo Clinic within 24 hours of draw, and by 10 am on Friday.

REFERENCE RANGE: Reference ranges are included with report.
REFERRAL LABORATORY:

See Mayo Clinic - Search Test Code 60591

LAST UPDATED:

9-16-2014

TEST SYNONYM(S):T-Cell or B-Cell Functional Assay

Methylene Tetrahydrofolate Reductase Enzyme Analysis

CERNER / EPIC MNEMONIC: MTHF RED
POE DESCRIPTION: MTHF REDUCTASE
CPT CODE: 83519
TEST INFORMATION: See OMIM information
DAYS PERFORMED: Arrange with Genetics laboratory in advance.
TURNAROUND TIME: 3 weeks
SPECIAL INSTRUCTIONS: Always include a control blood.
CONTAINER TYPE: [Lavender top (EDTA) tube
SPECIMEN REQUIREMENTS: Blood
MINIMUM VOLUME: 10 mL
HANDLING INSTRUCTIONS: Maintain at 4°C (wet ice) for transport to the laboratory. Specimen must reach laboratory within 1 hour after draw.
REFERENCE RANGE: Range will be included in report.

Methylmalonic Acid, Urine

CERNER / EPIC MNEMONIC: U/METMALON
POE DESCRIPTION: METHYLMALONIC ACID, URINE
CPT CODE:

83921-90

TURNAROUND TIME: 1 week
CONTAINER TYPE:

Plastic urine container, no preservative or 24-h Bottle #1

SPECIMEN REQUIREMENTS: 24-hour or random urine
MINIMUM VOLUME: 15 mL aliquot; indicate total volume.
HANDLING INSTRUCTIONS: Refrigerate until sent to the laboratory.
REFERENCE RANGE:

See Specialty Labs - Search Test Code 3496U

TEST SYNONYM(S):MMA, Urine; Urine Methylmalonic Acid

Methyltetrahydrofolate Homocysteine Methyltransferase

CERNER / EPIC MNEMONIC: MTHF HOMOC
CPT CODE: 83519
TEST INFORMATION: See OMIM information
DAYS PERFORMED: Arrange with laboratory in advance.
TURNAROUND TIME: 4-6 weeks
CONTAINER TYPE: Sterile container with sterile normal saline or sterile fibroblast media
SPECIMEN REQUIREMENTS: Skin biopsy
MINIMUM VOLUME: 3 mm³
HANDLING INSTRUCTIONS: Transport to the laboratory immediately.
REFERENCE RANGE: Will be stated in report
TEST SYNONYM(S):Methionine Synthase

Mitochondrial DNA Analysis

CERNER / EPIC MNEMONIC: MELAS; MERRF; NARP; KSS
CPT CODE: 83896; 83898; 83898; 83898
DAYS PERFORMED: Mon-Fri, 0800-1500
TURNAROUND TIME: 2-3 weeks
SPECIAL INSTRUCTIONS: Before collecting specimen, contact Genetics Lab (312-942-6298) for required patient history form.
CONTAINER TYPE: Lavender top (EDTA) tube
SPECIMEN REQUIREMENTS: Blood
MINIMUM VOLUME: 3 mL
HANDLING INSTRUCTIONS: Leave specimen at room temperature and deliver to the laboratory as soon as possible.

Mucopolysaccharides (Screening), Urine

CERNER / EPIC MNEMONIC:

U/MPS

POE DESCRIPTION: URT MUCOPOLYSACCHARIDE SCREEN; U/MPS
CPT CODE:

83866-90

TEST INFORMATION:

Must provide date of birth

TURNAROUND TIME:

2 weeks

CONTAINER TYPE:

No preservative, sterile urine cup

SPECIMEN REQUIREMENTS:

Random urine, 15.0 mL

MINIMUM VOLUME:

7.0 mL

REFERENCE RANGE:

See Specialty Labs - Search Test Code S42770

LAST UPDATED:

2-10-2014

TEST SYNONYM(S):Urine Mucopolysaccharides, Screen

Muscle Biopsy

CPT CODE: 88307
TEST INFORMATION: Test includes routine morphologic diagnosis. Special tests as requested by physician.
DAYS PERFORMED: Mon-Fri, 0800-1400
TURNAROUND TIME: 48 hours to 7 days
SPECIAL INSTRUCTIONS: All biopsies must be scheduled 24 hours in advance with specialty pathologist. Pertinent clinical history and laboratory data must accompany specimens, as well as specific muscle site biopsied. Outside institutions should contact Client Serivce Representative (312-942-7958) for submission and transport protocol.
CONTAINER TYPE: Sterile specimen container
COLLECTION:

Routine histology, histochemistry, and electron microscopy:  Submit specimen fresh in saline-moistened gauze.  Deliver to Laboratory Receiving Station, Rm 508 Jelke immediately after tissue is removed.

SPECIMEN REQUIREMENTS: Skeletal muscle
REJECTION CRITERIA: Improper fixation, improper or discrepant labeling, O.R. specimens with invalid "barcode" ID label
MINIMUM VOLUME: 10 mm x 10 mm x 10 mm segment of muscle
HANDLING INSTRUCTIONS: Deliver specimen to the laboratory immediately after removal.
ADDITIONAL INFORMATION:

Special studies (histochemistry and/or electron microscopy) are required for most other muscle diseases (mitochondrial, lipid, glycogen myopathies).  In certain diseases, an additional sample for enzyme assay or genetic analysis may be indicated; in this case, additional muscle should be snap frozen in the operating room.  Liquid nitrogen for freezing may be obtained in Rm 508 Jelke.

All muscle biopsies are sent to Dr. R. Wollman at U of C (773-702-6166)

Microsatellite Instability Analysis by PCR

CERNER / EPIC MNEMONIC:

MSI

POE DESCRIPTION:

MSI

CPT CODE:

83890, 83898, 83894, 83903, 83912

CDM NUMBER:

3391008, 3391006, 3391013, 3391021

DAYS PERFORMED:

Mon-Thur. 0900-1600; Fri. 0900-12:00

TURNAROUND TIME:

7-10 days

SPECIAL INSTRUCTIONS:

Must be accompanied by Specialty Tests (green) Requisition

CONTAINER TYPE:

SST tube

SPECIMEN REQUIREMENTS:

fixed embedded tumor and associated normal tissue

REJECTION CRITERIA:

tumor mass or normal tissue <4 sq. mm

MINIMUM VOLUME:

4-5 unstained thin sections of tumor and normal tissue; one H & E stained slide for tumor and for normal tissue

HANDLING INSTRUCTIONS:

Store slides at room temperature. Deliver to 240 B Rawson or order in SIS for pickup in Surgical Pathology

REFERENCE RANGE:

Instability in 1-5 markers

LIMITATIONS OF TEST:

Some fixing reagents may inhibit PCR amplification; small sample size <4 sq. mm may not be sufficient for analysis

METHODOLOGY:

Polymerase chain reaction; capillary electrophoresis with fluorescent detection

Methylenetetrahydrofolate Reductase C677T Mutation Analysis

CERNER / EPIC MNEMONIC: MTH C677T
POE DESCRIPTION:

MTHFR C677T Gene Mutation

CPT CODE: 83891, 83892, 83896 (x2), 83903 (x2), 83912
TURNAROUND TIME: 7-10 days
SPECIAL INSTRUCTIONS: Sample must be less than seven days old when received by commercial laboratory.
CONTAINER TYPE: Lavender top tube
SPECIMEN REQUIREMENTS: 2 mL EDTA whole blood
MINIMUM VOLUME: 1 mL whole blood
HANDLING INSTRUCTIONS: Sample should remain at room temperature.
REFERENCE RANGE: Normal
ADDITIONAL INFORMATION: Included as part of a Hypercoagulability Genetic Panel
TEST SYNONYM(S):MTH C677T; MTHFR C677T

Myoglobin

CERNER / EPIC MNEMONIC: MYO
POE DESCRIPTION: Myoglobin
CPT CODE: 83874
CDM NUMBER: 3181140
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Routine: 4 hours; STAT: 1 hour
CONTAINER TYPE:

Red top tube - 4 mL or SST tube

SPECIMEN REQUIREMENTS:

4.0 mL blood (2.0 mL serum)

MINIMUM VOLUME: 1.0 mL blood (0.3 mL serum)
REFERENCE RANGE: 0 - 110 ng/mL
METHODOLOGY: Chemiluminescent Microparticle Immunoassay (CMIA)
ADDITIONAL INFORMATION:

Myoglobin is present in both cardiac and skeletal muscle.  Any damage to either of these muscle types results in myoglobulin released into the blood stream.

Mycophenolate Mofetil

CERNER / EPIC MNEMONIC: MYCOPHENOL
POE DESCRIPTION:

MYCOPHENOL

CPT CODE:

80180-90

TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Lavender top (K2 EDTA) tube - 6 ml tube or Green top (lithium heparin) - 10 ml

SPECIMEN REQUIREMENTS:

6 mL blood (4 mL plasma)

MINIMUM VOLUME:

4 mL blood (2 mL plasma)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 4910

METHODOLOGY:

HPLC

LAST UPDATED:

11-11-2013

TEST SYNONYM(S):Mycophenolic Acid; Mofetil; Cellcept

Metanephrines, Plasma

CERNER / EPIC MNEMONIC: METANEP-PL
POE DESCRIPTION: METANEP
CPT CODE: 83835-90
TEST INFORMATION:

Includes metanephrines and normetanephrines

TURNAROUND TIME:

5-7 working days

SPECIAL INSTRUCTIONS: Patient should discontinue epinephrine and epinephrine-like drugs at least one week before obtaining the specimen.¿ The patient must refrain from using caffeine, medications, and tobacco, and from drinking coffee, tea, or alcoholic beverages for at least four hours before the specimen is drawn.
CONTAINER TYPE: Two 7 mL Lavender tops
SPECIMEN REQUIREMENTS: 8 mL EDTA whole blood (2.5 mL EDTA plasma)
MINIMUM VOLUME: 3.0 mL EDTA whole blood (1.5 mL EDTA plasma)
REFERENCE RANGE:

See Specialty Labs - Search Test Code S51767

METHODOLOGY: HPLC
TEST SYNONYM(S):Normetanephrines

Myeloma Profile (25)

CERNER / EPIC MNEMONIC:

Order individually

POE DESCRIPTION: MYELOMA PROFILE (25)
CPT CODE: 82232; 84165; 86334; 82784 (x3); 84165; 86334; 81050
TEST INFORMATION: Test includes B2 MICRO, IEP, QT IGS, U/IEP
DAYS PERFORMED:

Varies by test.

TURNAROUND TIME: Varies by test.
CONTAINER TYPE:

Red top tube or SST tube AND Plastic 24-hour urine container with no preservative (Chemistry bottle #1).

SPECIMEN REQUIREMENTS: Blood (serum) and urine.
MINIMUM VOLUME: 1.0 mL serum.
TEST SYNONYM(S):Myeloma Panel

Manganese,Whole Blood

CERNER / EPIC MNEMONIC: MN
POE DESCRIPTION: MANGANESE; MN
CPT CODE: 83785-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Dark blue top (EDTA) tube (Vacutainer® #369736) - or  Dark blue top (heparin) tube (Vacutainer® #369735)

SPECIMEN REQUIREMENTS: 2.0 mL EDTA whole blood
MINIMUM VOLUME:

1.0 mL EDTA whole blood

REFERENCE RANGE: See Specialty Labs - Search Test Code 4872W

TEST SYNONYM(S):Mn, Blood

Meconium (Stool) Drug Screen, Neonatal

CERNER / EPIC MNEMONIC: Q/DS
POE DESCRIPTION: DRUG SCREEN STOOL; STOOL DRUG SCREEN; FEC DRUG SCREEN; Q/DS
CPT CODE: 80100-90
TEST INFORMATION:

Includes testing for PCP, cocaine, opiates, marijuana, amphetamines and metamphetamines.

TURNAROUND TIME: 3-5 days
CONTAINER TYPE:

Sterile 80 mL urine container

SPECIMEN REQUIREMENTS:

Meconium from neonate. See attached instructions to collect, sample must be received from chain of custody form.

MINIMUM VOLUME:

2 grams

REFERENCE RANGE:

None detected

TEST SYNONYM(S):Drug Screen (Stool) Meconium, Neonatal

Mercury, Blood

CERNER / EPIC MNEMONIC: HG
POE DESCRIPTION: MERCURY; HG
CPT CODE: 83825-90
TURNAROUND TIME: 5-9 working days
SPECIAL INSTRUCTIONS:

Avoid seafood consumption 1 week prior to blood collection.

CONTAINER TYPE:

Dark blue top (EDTA) tube (Vacutainer® #369736) - or Dark blue top (heparin) tube (Vacutainer® #369735)

SPECIMEN REQUIREMENTS: 2.0 ml EDTA whole blood
MINIMUM VOLUME: 0.5 mL EDTA whole blood
REFERENCE RANGE: See Specialty Labs - Search Test Code 4873W

TEST SYNONYM(S):Hg, Blood

Mercury, Urine

CERNER / EPIC MNEMONIC: U/HG
POE DESCRIPTION: URT HG; U/MERCURY/24 HOUR; U/HG
CPT CODE: 83825-90, 81050
TURNAROUND TIME: 5-7 working days
SPECIAL INSTRUCTIONS:

Avoid seafood consumption for 48 hours prior to sample collection.

CONTAINER TYPE:

24-hour urine bottle, Chemistry bottle #5 (acid washed container)

SPECIMEN REQUIREMENTS: 24-hour urine
MINIMUM VOLUME: Submit entire urine collection to the laboratory.
HANDLING INSTRUCTIONS: Refrigerate during collection
REFERENCE RANGE: See Specialty Labs - Search Test Code 4873U

TEST SYNONYM(S):Hg, Urine; Urine Mercury

Metanephrines, Fractionated, Urine

CERNER / EPIC MNEMONIC: U/MET
POE DESCRIPTION: U/MET
CPT CODE:

82570-90, 83835-90

TEST INFORMATION:

Test includes urine total metanephrines, normetanephrines, metanephrines, creatinine and metanephrine/creatinine ratio.

TURNAROUND TIME: 5-7 working days
SPECIAL INSTRUCTIONS:

If possible, patient should discontinue all drugs at least one week prior to collection.  Medications known to interfere with this assay include: Alpha-methyldopa (Aldomet), Isoproterenol, Labetalol, Mandelamine, Metaclopramide, Acetaminophen (high concentrations only), Cimetidine, and Catecholamine-containing drugs, MAO inhibitors, diuretics, vasodilators.  Other interfering substances include smoking and drinking tea and coffee within four hours of collecting specimen.

CONTAINER TYPE:

24-hour urine bottle, Chemistry bottle #1

SPECIMEN REQUIREMENTS: 24-hour urine
MINIMUM VOLUME: Submit entire urine collection to the laboratory.
HANDLING INSTRUCTIONS: Refrigerate during collection
REFERENCE RANGE: See Specialty Labs - Search Test Code 3315U

LAST UPDATED:

3-11-2014

TEST SYNONYM(S):urine normetanephrines; Urine Metanephrines

Methanol, Blood

CERNER / EPIC MNEMONIC: VOL
CPT CODE: 84600-90
TURNAROUND TIME: 6-8 hours
SPECIAL INSTRUCTIONS:

Do not use alcohol solutions as a skin preparaton for collecting specimens.

CONTAINER TYPE: Gray top (sodium fluoride) tube
SPECIMEN REQUIREMENTS: 5 mL fluoridated whole blood
MINIMUM VOLUME:

0.5 mL fluoridated whole blood

REFERENCE RANGE:

See Quest Diagnostics Lab - Search Test Code 7240

TEST SYNONYM(S):Methyl Alcohol; Wood Alcohol

Mitochondrial Antibodies

CERNER / EPIC MNEMONIC: AMA
POE DESCRIPTION: MITOCHONDRIAL AB; AMA
CPT CODE:

83516-90

TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Red top tube or SST tube

SPECIMEN REQUIREMENTS: 2.0 mL blood (1.0 mL serum)
MINIMUM VOLUME: 1.0 mL blood (0.5 mL serum)
REFERENCE RANGE: See Specialty Labs  - Search Test Code 1101

TEST SYNONYM(S):Antimitochondrial Antibodies; AMA

Myelin Basic Protein, CSF

CERNER / EPIC MNEMONIC: MB/PRO
POE DESCRIPTION: MYELIN BASIC PROTEIN; MB/PRO
CPT CODE: 83873-90
TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Clear, sterile container

SPECIMEN REQUIREMENTS: 2.0 mL cerebrospinal fluid
MINIMUM VOLUME: 0.8 mL cerebrospinal fluid
REFERENCE RANGE: See Specialty Labs - Search Test Code 3900

Myocardial Antibodies

CERNER / EPIC MNEMONIC: MYOCARD AB
POE DESCRIPTION: MYOCARDIAL AB SCREEN
CPT CODE:

86255-90

TURNAROUND TIME: 5-7 working days
CONTAINER TYPE:

Red top tube or SST tube

SPECIMEN REQUIREMENTS:

4.0 mL blood 2.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.5 mL serum)

REFERENCE RANGE: See Specialty Labs - Search Test Code 1103

TEST SYNONYM(S):Antimyocardial Antibodies; Heart Antibodies; Anti-myofibrillar Antibody; Anti-sarcolemma Antibody

Myoglobin, Quantitative, Urine

CERNER / EPIC MNEMONIC: RU/MYO
POE DESCRIPTION: URR MYOGLOBIN; RU/MYO
CPT CODE: 83874-90
TEST INFORMATION:

Myoglobin is unstable in urine.

TURNAROUND TIME: 5-7 working days
SPECIAL INSTRUCTIONS:

Call Core Lab (x25002) for Myoglobin Transport Tube

CONTAINER TYPE:

Myoglobin Transport Tube

SPECIMEN REQUIREMENTS:

Random urine, 4 mL

REJECTION CRITERIA:

Urine not submitted in the Myoglobin Transport Tube from Specialty Labs.

MINIMUM VOLUME:

4.0 mL

REFERENCE RANGE:

See Specialty Labs - Search Test Code 661

LAST UPDATED:

8-7-2014

TEST SYNONYM(S):Urine Myoglobin

Mycobacterium Tuberculosis Direct Test

CERNER / EPIC MNEMONIC:

AFB MTD

POE DESCRIPTION:

AFB MTD

CPT CODE: 87556
CDM NUMBER: 3421131
TEST INFORMATION:

Detection of presence (positive) or lack of presence (negative) of Mycobacterium tuberculosis complex rRNA.

[Note: The Gen-Probe¿ amplification system (MTD) is specific for, but does not differentiate among, members of the M. tuberculosis complex.]

DAYS PERFORMED:

Daily.  Sent to IDPH if AFB Smear is positive.

TURNAROUND TIME:

3-5 days

SPECIAL INSTRUCTIONS:

Transport specimen to laboratory immediately.  If delay, store specimen at 2°-8°C.

CONTAINER TYPE: Sealed sterile container, no preservative
SPECIMEN REQUIREMENTS:

Undigested sputum (induced or expectorated), Bronchial specimens (bronchoalveolar lavage or bronchial aspirate), tracheal aspirate, pleural fluids.

REJECTION CRITERIA:

1. Specimen other than sputum (induced or expectorated), bronchial specimens (bronchoalveolar lavages or bronchial aspirates), tracheal aspirates or pleural fluids.

2. Specimen from previous MTD test 'positive' (without written justification from physician).

3. Patient specimens with a ''negative'' MTD result will only be tested three times in a 90-day period.

4. Specimens not stored at 2° to 8°C before transport.

5. Specimens older than 7 days.

6. Specimen not labeled.

7. Grossly bloody specimen.

MINIMUM VOLUME:

0.5 mL

REFERENCE RANGE: Not detected.
METHODOLOGY: The Gen-Probe¿ amplification system amplifies a specific ribosomal RNA (rRNA) target via DNA intermediates.¿ Detection of the amplified rRNA sequence (amplicon) is achieved using nucleic acid hybridization.¿ The chemiluminescent single-stranded DNA probe is complementary to the amplicon.¿ The labeled DNA probe combines with the amplified ribosomal RNA to form a stable RNA:DNA hybrid.¿ The selection reagent differentiates hybridized from non-hybridized probe.¿ The labeled RNA:DNA hybrids are measured in a luminometer.
REFERRAL LABORATORY:

Commercial Laboratory

ADDITIONAL INFORMATION:

Patients with respiratory specimens that test AFB smear positive will require on AFB MTD test.

LAST UPDATED:

5-27-2014

TEST SYNONYM(S):Mybacterium Tuberculosis Direct Test, Amplified (MTD

Methylenetetrahydrofolate Reductase A1298C Mutation

CERNER / EPIC MNEMONIC: MTH A1298C
POE DESCRIPTION: MTHFR A1298C Mutation
CPT CODE: 83890, 83892 (x2), 83896 (x4), 83903, 83912
TURNAROUND TIME: 7-10 days
CONTAINER TYPE:

Lavender top tube

SPECIMEN REQUIREMENTS: 7 mL EDTA whole blood
HANDLING INSTRUCTIONS: Sample must remain at room temperature.
REFERENCE CHART: Normal
METHODOLOGY: FRET
ADDITIONAL INFORMATION: The combination of a heterozygous MTHFR A1298C with a heterozygous MTHFR C677T mutation confers an increase in plasma homocyteine which is an independent risk factor for arterial and venous thrombosis.
TEST SYNONYM(S):MTHFR A1298C; MTH A1298C

Methylmalonic Acid, blood

CERNER / EPIC MNEMONIC:

METHYLMALO

POE DESCRIPTION:

METHYLMALONIC ACID

CPT CODE:

83921-90

CDM NUMBER:

3181387

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE: Red top tube
SPECIMEN REQUIREMENTS:

6.0 mL blood (3.0 mL serum)

MINIMUM VOLUME:

4.0 mL blood (2.0 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code 3496

METHODOLOGY:

Tandem mass spectrometry

Maple tree (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

MAPLE

POE DESCRIPTION:

Maple (Box Elder) Allergen

CPT CODE:

86003-90

TEST INFORMATION:

Included in the Rast Inhalant Panel

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.5 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code S51920

TEST SYNONYM(S):Box elder allergen

Meadow fescue grass (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

FESCUE

POE DESCRIPTION:

Meadow fescue allergen

CPT CODE:

86003-90

TEST INFORMATION:

Included in the Rast Inhalant Panel.

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code G4

Milk (cow''s) (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

MILK

POE DESCRIPTION:

Milk allergen (Cow)

CPT CODE:

86003-90

TEST INFORMATION:

Included in the Rast Food Panel

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs - Search Test Code F2

Mussel (IgE) Rast Allergen

CERNER / EPIC MNEMONIC:

MUSSEL

POE DESCRIPTION:

Mussel allergen

CPT CODE:

86003-90

TURNAROUND TIME:

5-7 working days

CONTAINER TYPE:

SST tube or red top

SPECIMEN REQUIREMENTS:

2.0 mL blood (1.0 mL serum)

MINIMUM VOLUME:

1.0 mL blood (0.3 mL serum)

REFERENCE RANGE:

See Specialty Labs  - Search Test Code F37