Parvovirus B19 PCR

CERNER / EPIC MNEMONIC:

PARVO DNA

CPT CODE:

87798

TEST INFORMATION:

DNA testing in conjunction with B19 specific serology provides the most reliable evidence of a recent infection.

DAYS PERFORMED:

Monday - Sunday

TURNAROUND TIME:

2-4 days

CONTAINER TYPE:

Collect plasma in sterile tube containing EDTA or ACD as anticoagulant. Whole Blood or bone marrow in sterile tubes containing EDTA or ACD as anticoagulant. Collect amniotic fluid and  tissue in sterile container. Collect serum in SST

SPECIMEN REQUIREMENTS:

1 mL plasma, sera, amniotic fluid, whole blood or bone marrow

REJECTION CRITERIA:

CSF or Synovial fluid

MINIMUM VOLUME:

0.5 mL

HANDLING INSTRUCTIONS:

Plasma, sera, whole blood, bone marrow, and amniotic fluid store and transport refrigerated.  Store and ship tissue frozen.

REFERENCE RANGE:

Not detected

METHODOLOGY:

Real-Time Polymerase Chain Reaction (RT-PCR)

REFERRAL LABORATORY:

Quest, Focus Diagnostics

ADDITIONAL INFORMATION:

Parvovirus B19 infection causes "Fifth Disease," a rash illness of childhood characterized by a bright red "slapped cheek"  rash and a lacy rash on the trunk and limbs. Joint pain and swelling is more common in adults. Individuals with chronic hemolytic anemia such as sickle cell anemia may develop transient aplastic crisis with severe anemia.  Immunocompromised individuals  may develop chronic or recurrent bone marrow suppression. Infection during pregnancy can lead to fetal hydrops and spontaneous abortion.

LAST UPDATED:

9-11-2013