Culture for Chlamydia

CERNER / EPIC MNEMONIC:

CHLAM CUL

CPT CODE:

87110, 87140

TEST INFORMATION:

Use to establish the diagnosis of Chlamydia trachomatis infection in suspected cases of conjunctivitis,  pneumonitis, urethritis, proctitis and cervicitis.  Test includes tissue culture technique for the isolation of Chlamydia trachomatis. Identification of inclusions with monoclonal antibody.

DAYS PERFORMED:

Monday - Saturday

TURNAROUND TIME:

4 -5 days

CONTAINER TYPE:

Viral transport medium (available from Clinical Microbiology Laboratory)

COLLECTION:

Obtain the viral transport medium from the Clinical Microbiology laboratory. Wipe the epithelial cell surface to remove any excess mucus. Discard the swab. Using a second swab, vigorously swab the site to be cultured and  place the swab directly into the viral transport medium. Place tissue and/or aspirate specimens directly into the viral transport medium.

SPECIMEN REQUIREMENTS:

Conjunctival swab, posterior nasopharyngeal aspirate, throat swab, cervical swab, urethral swab, tissue, sputum, lymph node aspirate and rectal swab without feces.

REJECTION CRITERIA:

Specimen not transported in viral transport medium, specimen heavily contaminated with bacteria, inappropriate specimen source, specimen collected with a wooden shaft or calcium alginate swab, tissues in formalin or other fixatives.

MINIMUM VOLUME:

1mL or swab submitted in viral transport medium.

HANDLING INSTRUCTIONS:

Specimen should be transported as soon as possible. When transportation is delayed refrigerate for 48 hours, or freeze at -70 degrees C.  Ship frozen.

REFERENCE RANGE:

No Chlamydia isolated

LIMITATIONS OF TEST:

The use of viral transport medium is essential to successful culture.

METHODOLOGY:

Tissue culture and direct fluorescent antibody (DFA).

REFERRAL LABORATORY:

Quest Diagnostics

ADDITIONAL INFORMATION:

Chlamydia infects the columnar epithelial cells and will not be recovered from squamous epithelial cells or inflammatory cells. In obtaining the specimen, clean the area of inflammatory cells and then  attempt to scrape epithelial cells for culturing. Patients in whom Chlamydia trachomatis is isolated are reported to the City of Chicago Board of Health. This is mandated by law.

LAST UPDATED:

9-11-2013

TEST SYNONYM(S):Chlamydia trachomatis culture; Lymphogranuloma Venereum Culture