Coccidioides Quantitative Antigen EIA

CERNER / EPIC MNEMONIC:

COCCI  AG

CPT CODE:

87449

CDM NUMBER:

3181503

TEST INFORMATION:

Intended use is as an aid in diagnosis of  coccidioidomycosis or to monitor therapy.

DAYS PERFORMED:

Test performed  Monday - Friday only.

TURNAROUND TIME:

2 - 7 days

CONTAINER TYPE:

Sealed sterile urine container (no preservative); red top Vacutainer tube or SST tube; sealed sterile cerebrospinal fluid collection tube; sealed sterile sputum or Leuken's container.

SPECIMEN REQUIREMENTS:

Urine, serum separated from the clot, plasma separated from the red cell layer, CSF, bronchial, or other sterile body fluid

REJECTION CRITERIA:

Inadequate specimen volume, particulate matter or viscosity that would not allow the specimen to be pipetted.

MINIMUM VOLUME:

2.0 mL

HANDLING INSTRUCTIONS:

Store in refrigerator if not sent the same day as obtained.

REFERENCE RANGE:

Negative = None Detected, Low Positive = 0.1-1.2 ng/ml, Moderate Positive = 1.3-8.2 ng/ml, High Positive = Above the Limit of Quantification

LIMITATIONS OF TEST:

Cross-reactions occur in histoplasmosis and possibly blastomycosis.  Sputolysin and NaOH treatment degrade the analyte detected in the assay.  Negative results do not exclude coccidioidomycosis:  testing both urine and serum offers the highest sensitivity.

METHODOLOGY:

Quantitative sandwich enzyme immunoassay (EIA)

REFERRAL LABORATORY:

Commercial Laboratory