B-Raf V600E Mutation Detection by SSP-PCR

CERNER / EPIC MNEMONIC:

BRAFPCR

CPT CODE:

83891, 83898, 83894, 83903, 83912

CDM NUMBER:

3391019, 3391013, 3391006, 3391021, 3391014

DAYS PERFORMED:

Mon-Thur., 0900-1600; Fri., 0900-1200

TURNAROUND TIME:

7-10 days

SPECIAL INSTRUCTIONS:

Specimens must be accompanied by a Specialty Tests requisition form

CONTAINER TYPE:

Fixed tissue block, or thin sections

SPECIMEN REQUIREMENTS:

2-3 ml bone marrow, 3-5 ml peripheral blood

REJECTION CRITERIA:

Unlabeled or mixed specimens, specimens mixed with more than 60% normal cells

MINIMUM VOLUME:

4 mm2 tumor tissue (5 micron sections); at least 40% tumor cells    

HANDLING INSTRUCTIONS:

Hold at room temperature, deliver to 1188 Jelke within 24 hours

REFERENCE RANGE:

Mutation detected/not detected

LIMITATIONS OF TEST:

Only detects the B-raf V600E mutation

METHODOLOGY:

Sequence specific PCR

ADDITIONAL INFORMATION:

May be used before K-Ras testing