Respiratory Virus PCR

CERNER / EPIC MNEMONIC:

RESVIRPCR

CPT CODE:

83900, 83901 x 6

TEST INFORMATION:

This test is used to detect the presence of RNA from Influenza A, Influenza B, Respiratory Syncytial Virus, Parainfluenza viruses 1, 2, 3 and 4, and Adenovirus.

DAYS PERFORMED:

Monday through Saturday during  respiratory virus infection season

TURNAROUND TIME:

24- 48 hours

SPECIAL INSTRUCTIONS:

Specimen must be received by the Clinical Microbiology Laboratory no later than 6:00am on the day of testing for same day result.

CONTAINER TYPE:

Nasopharyngeal swabs and throat swabs should be placed in M4 viral transport media.  Nasopharyngeal aspirates, nasopharyngeal washes, bronchoalveolar lavage, sputum, pleural fluid, transtracheal biopsy, transbroncheal biopsy, or lung biopsy should be placed in a sterile container.

SPECIMEN REQUIREMENTS:

Nasopharyngeal swab, nasopharyngeal aspirate, nasopharyngeal washes, throat swabs, bronchoalveolar labage, sputum pleural fluid, transtracheal biopsy, transbroncheal biopsy, lung biopsy

REJECTION CRITERIA:

Inappropriate specimen, improper collection, inappropriate specimen container, improper storage, unlabeled specimen, mislabeled specimen.

MINIMUM VOLUME:

1 ml

HANDLING INSTRUCTIONS:

Specimens should be transported to the laboratory as soon as possible.  When transportation is delayed, refrigerate the specimen

REFERENCE RANGE:

This assay will be reported as 'RNA detected', 'No RNA detected', or 'Equivocal' for each respiratory virus tested.  Equivocal results are values that fall between +/- 10% of the positive cutoff value.  If an equivocal test result is obtained, another specimen should be submitted if a respiratory virus infection is still suspected.

LIMITATIONS OF TEST:

A negative result does not exlude the possibility of respiratory virus infection since very low levels of infection or sampling error may cause a false negative result.  The assay does not detect respiratory viruses other than those listed above.

METHODOLOGY:

Polymerase Chain Reaction with detection by Lumenix technology