|CERNER / EPIC MNEMONIC:||
This test includes detection of Varicella-zoster virus. Varicella-zoster virus in CSF.
Monday, Wednesday, and Friday
Maximum of 72 hours
Specimen should be received by the laboratory as soon as possible.
CSF collection tube ; M4 transport medium for swabs
Vesicular lesions: Wash the surface of the vesicles with sterile saline. Carefully open several vesicles and soak up the vesicular fluid with a clean sterile swab. The specimen should be collected in the first three days of eruption. Place the swab into M4 transport medium immediately after collection.
1 ml of CSF in a sterile tube from the lumbar puncture kit
Dry swab; swab specimen not in M4 transport media; specimen not refrigerated prior to transport; specimen not received in a sterile sealed container; specimen not closed properly and leaked into transport bag; excessive delay in transport; inappropriate specimen container; specimen unlabeled or mislabeled.
1 mL of CSF or one swab in M4 transport media
The specimen should be transported to the lab as soon as possible. When transportation is delayed, refrigerate.
No VZV detected.
|LIMITATIONS OF TEST:||
Reliable results are dependent on appropriate specimen collection, transport storage, and processing procedures. No detection limits have been established for other specimen types or transport media.
Isolation of target and control DNA, real time PCR with detection by target specific probes for VZV and internal control.
The detection of Varicella-Zoster virus DNA is based upon the real-time amplification and detection of specific VZV genomic DNA sequences by PCR from total DNA extracted from the specimen. Probes specific for VZV are used to identify and differentiate the products of the PCR amplification.
|TEST SYNONYM(S):||VZV detection by real time PCR; VZV PCR; Varicella zoster virus by real time PCR; Herpes zoster|