|CERNER / EPIC MNEMONIC:||
87529 x 2
This test includes detection of HSV-1 and HSV-2. Herpes simplex virus detected in CSF. Herpes simplex identified in a child < 1 year old. Detects HSV-1 and HSV-2 in skin and genital specimens.
Monday, Wednesday, and Friday
Maximum of 72 hours
Specimen should be received by the laboratory as soon as possible.
CSF collection tube ; M4 transport media for swabs
Specimens should be collected in the acute stage of the disease, preferably within 3 days and no longer than 7 days after onset of illness. Specimens should be collected on a sterile swab and placed into M4 transport medium immediately after collection.
Specimen depends upon the type of infection:
Specimen not received in a sterile sealed container; specimen not closed properly and leaked into transport container; excessive delay in transport; inappropriate specimen container; specimen unlabeled or mislabled.
1 mL of CSF or one swab in M4 transport media
The specimen should be transported to the laboratory as soon as possible. When transportation is delayed, refrigerate.
No HSV-1 or HSV-2 detected
|LIMITATIONS OF TEST:||
Reliable results are dependent on appropriate specimen collection, transport, storage, and processing procedures. No detection limits have been established for other specimen types or transport media.
Isolation of target DNA, real-time PCR with detection by target specific probes for HSV-1 and HSV-2.
In house studies have shown the analytical sensitivity of this assay is 10 copies/PCR reaction and 250 copies/patient specimen. The genes that are amplified and detected in this assay are surface glycopeptide gG (HSV-1) and gD (HSV-2).
|TEST SYNONYM(S):||HSV 1 & 2 detection by real time PCR; Herpes simplex virus by real time PCR; Herpes simplex|