Jak2 V617F Mutation Detection by SSP-PCR

CERNER / EPIC MNEMONIC:

JAK2

CPT CODE:

83890, 83898, 83894, 83914, 83912

DAYS PERFORMED:

Monday-Thursday, 0900-1600; Fri., 0900-1200

TURNAROUND TIME:

7-10 days

SPECIAL INSTRUCTIONS:

Specimens must be accompanied by a Specialty Tests requisition form.

CONTAINER TYPE:

ACD or EDTA

SPECIMEN REQUIREMENTS:

2-3 mL bone marrow, 3-5 mL peripheral blood

REJECTION CRITERIA:

Unlabeled or mixed specimens

MINIMUM VOLUME:

1 mL blood, 0.5 mL bone marrow

HANDLING INSTRUCTIONS:

Hold at room temperature, deliver to 1188 Jelke within 24 hours

REFERENCE RANGE:

Mutation detected/not detected

LIMITATIONS OF TEST:

Only detects the Jak2 V617F mutation

METHODOLOGY:

Sequence specific PCR