Lymphoma and Leukemia Gene Rearrangement, Ig Light Chain Gene Rearrangement by PCR

CERNER / EPIC MNEMONIC: LCPCR
POE DESCRIPTION: LCPCR
CPT CODE: 83891, 83894, 83898, 83912, 83902, 83903 , 83898
CDM NUMBER: 3391014, 3391018, 3391013, 3391006, 3391021, 3391019
TEST INFORMATION: Detects monoclonal B cell population by IgL kappa and IgL lambda gene rearrangement
DAYS PERFORMED: Mon. - Thurs. 9:00-4:00; Fri. 9:00-12:00
TURNAROUND TIME: 14 days
SPECIAL INSTRUCTIONS: Fresh blood or bone marrow
CONTAINER TYPE: ACD tube (yellow top) or EDTA tube (purple top)
COLLECTION: Venipuncture
SPECIMEN REQUIREMENTS: 3-5 ml bone marrow; 5-10 ml blood
MINIMUM VOLUME: 2 ml bone marrow; 3 ml blood
HANDLING INSTRUCTIONS: Deliver to Cellular Laboratory (1188 Jelke) within 48 hours, hold at room temperature
REFERENCE RANGE: No rearrangement observed; rearrangement observed
LIMITATIONS OF TEST: Detects 50% of possible IgL kappa and lambda gene rearrangements
METHODOLOGY: Reverse transcriptase polymerase chain reaction (RT-PCR) and detection by heteroduplex analysis