Microsatellite Instability Analysis by PCR

CERNER / EPIC MNEMONIC:

MSI

POE DESCRIPTION:

MSI

CPT CODE:

83890, 83898, 83894, 83903, 83912

CDM NUMBER:

3391008, 3391006, 3391013, 3391021

DAYS PERFORMED:

Mon-Thur. 0900-1600; Fri. 0900-12:00

TURNAROUND TIME:

7-10 days

SPECIAL INSTRUCTIONS:

Must be accompanied by Specialty Tests (green) Requisition

CONTAINER TYPE:

SST tube

SPECIMEN REQUIREMENTS:

fixed embedded tumor and associated normal tissue

REJECTION CRITERIA:

tumor mass or normal tissue <4 sq. mm

MINIMUM VOLUME:

4-5 unstained thin sections of tumor and normal tissue; one H & E stained slide for tumor and for normal tissue

HANDLING INSTRUCTIONS:

Store slides at room temperature. Deliver to 240 B Rawson or order in SIS for pickup in Surgical Pathology

REFERENCE RANGE:

Instability in 1-5 markers

LIMITATIONS OF TEST:

Some fixing reagents may inhibit PCR amplification; small sample size <4 sq. mm may not be sufficient for analysis

METHODOLOGY:

Polymerase chain reaction; capillary electrophoresis with fluorescent detection