DNA Analysis of Cells, Fresh or Frozen

CPT CODE: 88182
TEST INFORMATION: METHOD - Flow Cytometry
DAYS PERFORMED: Mon-Fri, 0800-1600
TURNAROUND TIME: 1 week
SPECIAL INSTRUCTIONS: All specimens should be delivered to Surgical Pathology
CONTAINER TYPE: 50 mL centrifuge tube for bladder barbotage, 15 mL centrifuge tube with 50% ethanol for cell scrapings.
SPECIMEN REQUIREMENTS: Bladder barbotage in normal saline solution or cells scraped from tumors with a scalpel
REJECTION CRITERIA: Inadequate amount of cells collected or absence of tumor cells in sample
MINIMUM VOLUME: 10,000 cells
HANDLING INSTRUCTIONS: Refrigerate
REFERENCE RANGE: Diploid (negative). If nondiploid (positive), a written interpretation will be included. S-phase for breast cancer given in relation to means at Rush.
METHODOLOGY: Propidium iodide staining by a modification of the Vindelov procesure and analysis by flow cytometry
TEST SYNONYM(S):DNA Content of Cells, Fresh or Frozen; Cell Cycle Analysis; Aneuploidy of Cells, Fresh or Frozen; DNA Aneuploidy of Cells, Fresh or Frozen; DNA Ploidy & S Phase of Cells, fresh or Frozen