Leukemia Gene Translocation, bcr-abl by Reverse Transcriptase PCR and Quantitative PCR (qPCR)

CERNER / EPIC MNEMONIC: BCRABL
POE DESCRIPTION: BCR-ABL TRANSLOCATION
CPT CODE: 83894; 83912; 83898 (x2); 83902; 83891
DAYS PERFORMED: Mon-Thur, 0900-1600; Fri, 0900-1200
TURNAROUND TIME: 7-10 days
CONTAINER TYPE: Yellow top (ACD) tube
SPECIMEN REQUIREMENTS: Blood or bone marrow.
MINIMUM VOLUME: 1-2 mL bone marrow; refer to lymphoma and Leukemia Gene Rearrangement test for minimum blood volume required.
HANDLING INSTRUCTIONS: Store ACD tubes at room temperature; deliver to the Cellular Laboratory within 24 hours.
REFERENCE RANGE: No translocation observed; (BCRABL/ABL + BCRABL) x 100 = 0.01-100
LIMITATIONS OF TEST:

May not detect rare translocation breakpoints

TEST SYNONYM(S):bcr Gene Translocation; Breakpoint Cluster Region Translocation; Chronic Myelogenous Leukemia; Philadelphia Chromosome Translocation