Lymphoma Gene Translocation, bc1-2 by Polymerase Chain Reaction (PCR)

CERNER / EPIC MNEMONIC: BCL2PCR
POE DESCRIPTION: BCL 2 GENE; BCL2
CPT CODE: 83898; 83898; 83912; 83894; 83891
DAYS PERFORMED: Mon-Thur, 0900-1600; Fri, 0900-1200
TURNAROUND TIME: 7-10 days
CONTAINER TYPE: Yellow top (ACD) tube for blood and bone marrow
SPECIMEN REQUIREMENTS: Biopsy, bone marrow, blood, body fluid. Note: Leftover specimen submitted to Pathology or Cellular Laboratory will be used whenever possible. Specimens submitted for the Lymphoma and Leukemia Gene Rearrangement test will be used to determine the presence of an amplifiable bc1-2 translocation.
MINIMUM VOLUME: The bcl-2 translocation test by PCR may be ordered alone; in this case the sample quantity required is 2-3 mL of blood and 1-2 mL of bone marrow.
HANDLING INSTRUCTIONS: Store yellow top (ACD) tubes at room temperature; deliver to the Cellular Laboratory within 48 hours; 24 hours for quantitative PCR
REFERENCE RANGE: No translocation observed; 0.01-100% of cells in specimen.
LIMITATIONS OF TEST:

Will detect 80% of translocation breakpoints

TEST SYNONYM(S):bc1-2 Translocation; Major Breakpopint Region (MBR) Translocation; Minor Cluster Region (MCR)