Wound/Infected Site, Routine Culture

CPT CODE: 87071; 87205
TEST INFORMATION: The test includes a direct Gram stain, isolation and identification of microorganisms, and susceptibility testing, if appropriate. Other isolated organisms (i.e. anaerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Gram stain: 2 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 24 hours. Culture reports of no growth will be issued after 72 hours. Completion of culture reports may take 3-5 days after receipt of the specimen when multiple isolates are found.
SPECIAL INSTRUCTIONS: The specific anatomic site of the specimen MUST be specified on the requisition with surface and/or deep wound delineated.
CONTAINER TYPE: Copan® II swab, sealed sterile container
COLLECTION: Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface organisms. Ideally, material is obtained by needle aspiration through intact surface, which has been cleaned with antiseptic, and injected into a sealed, sterile container. Sampling of open lesions is enhanced by deep aspiration using a sterile plastic catheter. If irrigation is necessary, nonbacteriostatic sterile saline may be used. Pulmonary samples may be obtained by transtracheal percutaneous needle aspiration. Optimal isolation of microorganisms from tissue is accomplished by processing as much tissue as possible.
SPECIMEN REQUIREMENTS: Pus or other material aseptically obtained from an infected site, abscess, or wound
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, specimen received in a preservative.
MINIMUM VOLUME: 0.5 mL or two swabs
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE: Gram stain: depends upon the site of the specimen.Culture: no growth.
LIMITATIONS OF TEST: A specimen with additional microbiologic procedures will be processed only if the specimen is of adequate quantity for all tests requested. A physician will be contacted to prioritize requested procedures when the specimen is of an inadequate quantity. The portion of the surgical specimen submitted for microbiologic analysis should be separated whenever possible from the portion submitted to the Pathology Department utilizing sterile technique.
METHODOLOGY: Smear: Gram stain.Culture: conventional culture on routine media.
TEST SYNONYM(S):Abscess Culture, Routine; Ear Culture, Routine; Eye Culture, Routine