Virus Culture

CERNER / EPIC MNEMONIC: C VIR
POE DESCRIPTION: CU VIRUS; C VIR
CPT CODE: 87252
TEST INFORMATION: The test includes inoculation of specific cell cultures for isolation of viral agents.
DAYS PERFORMED: Monday-Saturday, 24 hours
TURNAROUND TIME: Negative viral culture reports will be issued after 2 weeks. Negative viral culture reports for Cytomegalovirus will be issued after 3 weeks.
SPECIAL INSTRUCTIONS: Use a lavender top Vacutainer® (EDTA) tube to collect blood specimens. Obtain a viral transport medium from the Clinical Microbiology Laboratory. Place the non-blood specimen directly into the viral transport medium. Avoid using WOODEN swabs for specimen collection.
CONTAINER TYPE: Lavender top Vacutainer (EDTA) tube for blood, sterile sealed container, viral transport medium (available from Clinical Microbiology Laboratory)
COLLECTION: AVOID using wooden swabs.
SPECIMEN REQUIREMENTS: Blood, sputum, bronchial, nose or throat swabs, ocular swabs, cerebrospinal fluid, urine, biopsy, autopsy specimens, and stool. See table.
REJECTION CRITERIA: Improper storage or transport of specimen, gross specimen contamination, improper specimen collection, failure to use the viral transport medium, inappropriate Vacutainer® tube, wooden swab used for specimen collection.
MINIMUM VOLUME: 1 mL fluid, 0.5 gram solid material
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No virus isolated
LIMITATIONS OF TEST: Tissue culture may be negative in the presence of a viral infection.
METHODOLOGY: Tissue culture
TEST SYNONYM(S):Virus Identification; Virus Isolation