Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
5 mL blood (1 mL serum)
Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
Negative: Rubella, nonimmune; Toxoplasma gondii: not detected; CMV: not detected; Herpes simplex 1: not detected; Herpes simplex 2: not detected
Microparticle enzyme immunoassay (MEIA) and enzyme immunoassay (EIA)
The test is used to screen for IgG antibodies to Toxoplasma gondii, Rubella, Cytomegalovirus, and Herpes simplex viruses. The test is important in newborn infants, and is used to evaluate possible congenital infection. Repeat positive test in 10-14 days and monthly as indicated. If TORCH syndrome is suspected in newborns, individual IgM antibody tests for each of the above antigens should be evaluated and serial specimens tested for changing IgG titers. Toxoplasmosis is reported as IU/mL (International Units); Cytomegalovirus is reported as AU/mL (Antibody Units); Herpes 1 and 2 are reported as REV (Relative EIA values). The cut-off values for positive assays are as follows: Toxoplasma gondii IgG: >6 IU/mL; Cytomegalovirus IgG: >15 AU/mL; Herpes simplex 1: >1 REV; Herpes simplex 2: >1 REV