Respiratory Syncytial Virus Culture and Antigen

CERNER / EPIC MNEMONIC: RSV AG; C VIR
POE DESCRIPTION: RSV ANTIGEN DETECTION
CPT CODE: 87420; 87252
CDM NUMBER:

 

TEST INFORMATION: The test includes a direct screening test for RSV antigen. A viral culture is perfomed on all RSV antigen screens.
DAYS PERFORMED: Monday-Saturday, 0700-1400. During respiratory viral season, the assay is perfomed Monday-Friday at 0830, 1330, 1930, and 0230; Saturday and Sunday at 0230, 1330, and 1930.
TURNAROUND TIME: Antigen detection: same day providing the specimen is received by 1330 or same day during respiratory viral season. Culture: 7 days.
CONTAINER TYPE: Viral transport medium
COLLECTION:

Introduce 3-7 cc sterile saline into the patient's posterior nasal cavity and immediately aspirate the fluid via vacuum suction and Leuken's trap. Specimens must then be placed into the viral transport medium (available from the Clinical Microbiology Laboratory).

SPECIMEN REQUIREMENTS: Nasopharyngeal washes and secretions
REJECTION CRITERIA: Inappropriate specimen container, specimen NOT submitted in viral transport medium, specimen NOT refrigerated during transport, excessive delay in transport.
MINIMUM VOLUME: 1 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate. Keep specimen cold and moist. Do NOT freeze specimen.
REFERENCE RANGE: No RSV antigen detected; No virus isolated.
LIMITATIONS OF TEST: Respiratory syncytial virus is extremely labile.
METHODOLOGY: Direct antigen detection: Enzyme-linked immunosorbent assay (ELISA).Culture: Inoculation of specimens into cell cultures, incubation, observation for characteristic cytopathic effect in 2-7 days, and identification by fluorescent monoclonal antibodies specific for Respiratory Syncytial Virus.
ADDITIONAL INFORMATION: Serology is also available to detect antibodies to Respiratory Syncytial Virus.
TEST SYNONYM(S):RSV Culture