Culture for Legionella pneumophila

POE DESCRIPTION:

C LEGIONSM

CPT CODE:

87081, 87015

TEST INFORMATION:

The test includes culture for Legionella.

DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME:

Preliminary cultures reports are available after 48 hours. Culture reports of no growth will be issued after 5 days. Completion of culture reports may take 6-7 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.

CONTAINER TYPE: Sealed sterile container; Leuken`s tube
COLLECTION: Specimens are to be collected from a site prepared utilizing aseptic technique. Non-bacteriostatic sterile saline should be used when obtaining washings and/or lavage specimens. Material obtained by needle aspiration (transbronchial lung biopsy) is injected into a sealed sterile container.
SPECIMEN REQUIREMENTS: Lung tissue, other body tissue, pleural fluid, other body fluid, transtracheal aspiration, bronchoalveolar lavage, BAL, and bronchial brushing.
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME: 2 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE:

No Legionella species isolated

LIMITATIONS OF TEST:

The sensitivity of the culture is 50-80%; however, the specificity is 100%.

METHODOLOGY:

Culture: conventional culture using routine media and media selective for Legionella isolation.

ADDITIONAL INFORMATION: If clinical suspicion of Legionella is high, several specimens may need to be submitted since small numbers of organisms may be present. Legionella species are widespread in the environment and are associated almost exclusively with surface and potable waters or moist environments. Click here for more information about Legionnaire''s disease.
LAST UPDATED:

5-20-2014