Isospora Diagnostic Procedure, Stool

CERNER / EPIC MNEMONIC: SM OOCYST
POE DESCRIPTION: SM CRYPTOSPORIDIUM
CPT CODE: 87207; 87015
TEST INFORMATION: Test includes examination of the stool for the presence of Cryptosporidium, Isospora, and Cyclospora by a modified acid-fast stain.
DAYS PERFORMED: Monday-Saturday, 0700-1400
TURNAROUND TIME: 1-2 days
CONTAINER TYPE: Plastic feces specimen container, no preservative; sealed container or nonsterile clean container
COLLECTION: The specimen should be collected directly into the plastic feces specimen container (no preservative) or into a bedpan, avoiding contamination with urine or water. Transfer the feces from the bedpan into the plastic feces container or a sealed container.
SPECIMEN REQUIREMENTS: Fresh stool
REJECTION CRITERIA: Specimen on outside of container, insufficient quantity of specimen, rectal swab, inappropriate specimen, specimen submitted in a preservative, specimen contaminated with water and/or urine.
MINIMUM VOLUME: 1 mL or 1 gram
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No Isospora, Cryptosporidium, or Cyclospora detected
LIMITATIONS OF TEST: Organisms are most readily demonstrated in diarrheal stools.
METHODOLOGY: Modified acid-fast stain of a concentrated specimen on air-dried methanol fixed smears (decolorization with 1% sulfuric acid).
ADDITIONAL INFORMATION: If this test procedure is requested on a specimen other than stool, a consultation with the Director or a Supervisor is recommended. Click here for more information about Cryptosporidiasis, Isosporasis, and Cyclosporiasis