Hepatitis C Virus RNA Quantitation

CERNER / EPIC MNEMONIC: HCVRNA QT
POE DESCRIPTION:

HCVRNA QT

CPT CODE: 87522
CDM NUMBER: 3511006
TEST INFORMATION: The test includes quantitative detection of Hepatitis C virus RNA.
DAYS PERFORMED: Tuesday
TURNAROUND TIME: 1-7 days
SPECIAL INSTRUCTIONS:

Specimen must be received by the Microbiology laboratory no later than 0700 on the day the test is to be performed. This test cannot be added onto an existing molecular test.

CONTAINER TYPE:

 Red top Vacutainer® tube,  SST TM tube

SPECIMEN REQUIREMENTS:

Blood (serum)

REJECTION CRITERIA:

Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, specimen not frozen within 6 hours of collection, inappropriate specimen container, insufficient specimen volume, specimen not appropriately labeled.

MINIMUM VOLUME:

2 mL blood (1 mL serum)

HANDLING INSTRUCTIONS:

Store at 2 - 30C until received by the laboratory.  Serum must be separated from the clot  and frozen at -20C within 6 hours of specimen collection.

REFERENCE RANGE:

<12 IU/ml

LIMITATIONS OF TEST:

A specimen with a result of "<12 IU/ml" cannot be presumed to be negative for HCV RNA

METHODOLOGY:

Real Time polymerase chain reaction (RT-PCR)

ADDITIONAL INFORMATION:

Linearity of the assay is between 12 and 100,000,000 IU/ml

LAST UPDATED:

12-1-2011

TEST SYNONYM(S):Quantitative HCV RNA; HCV Quantitative; Hep C Viral Load