HIV RNA Quantitation

CERNER / EPIC MNEMONIC:

HIVRNAQNT

POE DESCRIPTION:

HIVRNAQNT

CPT CODE: 87536
CDM NUMBER:

3481077

DAYS PERFORMED:

Thursdays

TURNAROUND TIME: 1-7 days
SPECIAL INSTRUCTIONS:

Specimens received later than 0600 on the day the test is performed will be held for the next run.

CONTAINER TYPE:

Lavender top Vacutainer (EDTA) tube

SPECIMEN REQUIREMENTS: Blood (plasma)
REJECTION CRITERIA:

Inappropriate specimen container, clotted specimen, insufficient specimen volume, gross contamination of the specimen, plasma not separated within 6 hours of collection, missing or wrong patient identifers on the specimen.

MINIMUM VOLUME:

5 ml of whole blood (2 ml of plasma)

HANDLING INSTRUCTIONS:

Specimen must be transported to the lab as soon as possible.  The plasma MUST be removed from the cells within 6 hours of collection, missing or wrong patient identifiers on the specimen.

REFERENCE RANGE:

<40 copies/ml of HIV-1 RNA detected. 

LIMITATIONS OF TEST:

The assay is limited to the quantitation of HIV-1 RNA in human plasma.  Reliable results are dependent on appropriate specimen collection, transport, storage, and processing procedures.  This assay is not intended to be used as a screening test for HIV-1 or as a diagnostic test to confirm HIV-1 infection.

METHODOLOGY:

Real time polymerase chain reaction

ADDITIONAL INFORMATION:

Linearity of the assay 40 to 100,000,000 copies/ml

LAST UPDATED:

12-9-2011

TEST SYNONYM(S):HIV RNA Viral Load; HIV Quantitative