Gastric Aspirate, Routine

CERNER / EPIC MNEMONIC: C AERGS
POE DESCRIPTION: CU AEROBIC W GRAM STAIN; C AERGS
CPT CODE: 87071; 87205
TEST INFORMATION: The test includes a direct Gram stain, isolation and identification of microorganisms, and susceptibility testing, if appropriate.
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Gram stain: 2 hours after receipt of the specimen in the laboratory. Preliminary culture reports available after 24 hours. Culture reports of no growth will be issued after 72 hours. Completion of culture reports may take 3-4 days after receipt of the specimen when multiple isolates are found.
SPECIAL INSTRUCTIONS: The specific anatomic site of the specimen MUST be specified on the order or the requisition.
CONTAINER TYPE: Sealed sterile container, no preservative
COLLECTION: The patient should NOT have eaten within the previous 5 hours. The specimen of choice is an aspirate, NOT a swab. Specimens are to be collected via a nasogastric tube introduced orally or nasally to the stomach. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) organisms. Chilled, sterile, distilled water or nonbacteriostatic sterile saline should be used to perform the lavage. The specimen should be placed directly into the sterile container and the lid secured tightly. Contamination with normal flora from skin or other body sources MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
SPECIMEN REQUIREMENTS: Gastric aspirate
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, patient older than 5 years of age.
MINIMUM VOLUME: 1 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
METHODOLOGY: Conventional culture
ADDITIONAL INFORMATION: Gastric aspirates for aerobic bacteria are cultured from newborns ONLY. Gastric aspirates submitted for routine culture from patients older than 5 years of age are unacceptable and will NOT be cultured.