Gastric Aspirate Culture, Anaerobic

CERNER / EPIC MNEMONIC: C ANAEROBE
POE DESCRIPTION: CU ANAEROBIC; C ANAEROBE
CPT CODE: 87075
TEST INFORMATION: Test includes culture for anaerobic organisms. Other isolated organisms (i.e. aerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Cultures are examined every 48 hours. Preliminary culture reports available after 48 hours. Culture reports of no growth will be issued after 5 days. Completion of culture reports may take 7-10 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.
SPECIAL INSTRUCTIONS: Avoid exposure to atmospheric oxygen. Specimens should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. The specific anatomic site of the specimen MUST be specified on the order or requisition.
CONTAINER TYPE: BBLTM Port-A-Cul Transport System
COLLECTION: The patient should NOT have eaten within the previous 5 hours. The specimen of choice is an aspirate, NOT a swab. Specimens are to be collected via a nasogastric tube introduced orally or nasally to the stomach. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) anaerobes. Chilled, sterile, distilled water or nonbacteriostatic sterile saline should be used to perform the lavage. The specimen should be placed directly into the BBLTM Port-A-Cul Transport System and the lid secured tightly. Contamination with normal flora from skin or other body sources MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
SPECIMEN REQUIREMENTS: Gastric aspirate
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, patient older than 5 years of age.
MINIMUM VOLUME: 0.5-5mL, undiluted
HANDLING INSTRUCTIONS: Avoid exposure to atmospheric oxygen. Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. The specimen MUST be processed promptly since organisms die rapidly in gastric washings unless the sample is neutralized.
REFERENCE RANGE: No growth of anaerobic bacteria
LIMITATIONS OF TEST: A specimen received in anaerobic transport devices is not suitable for routine fungal or mycobacterial cultures. If more than three anaerobic isolates are present, the Bacteroides fragilis group, pigmented gram-negative rods (Prevotella and/or Porphyromonas species), Actinomyces species, and Clostridium perfringens will be the only isolates identified. Anaerobic susceptibility testing is NOT performed routinely. Should susceptibility testing be required, call the Clinical Microbiology laboratory at (312)942-5452. Cultures will NOT be performed on specimens from patients older than 5 years of age.
METHODOLOGY: Conventional culture utilizing media specifically formulated for anaerobic isolation.
ADDITIONAL INFORMATION: Gastric aspirates are frequently diluted with saline.
TEST SYNONYM(S):Anaerobic Culture, Gastric Aspirate