Cystic Fibrosis Culture, Sputum

CERNER / EPIC MNEMONIC: C CF PROT
CPT CODE: 87205, 87071
TEST INFORMATION: Test includes a direct Gram stain, inoculation of routine conventional and selective media, isolation and identification of microorganisms, and susceptibility testing, if appropriate.
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Gram stain: 2 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 24 hours. Cultures with no growth or normal flora only will be finalized after 7 days. Complete reports of cultures from which pathogens are isolated may take 7-10 days after receipt of the culture depending upon the nature of the microorganisms detected.
SPECIAL INSTRUCTIONS: Order or requisition must state CF patient.
CONTAINER TYPE: Sputum or Leuken`s container
SPECIMEN REQUIREMENTS: Sputum, Leuken`s, tracheal aspirate
REJECTION CRITERIA: Inappropriate specimen container, insufficient volume, inappropriate specimen source. Only one specimen will be accepted per day.
MINIMUM VOLUME: 1 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: Normal upper respiratory flora only
METHODOLOGY: Inoculation of routine and selective media for Haemophilus species, Staphylococcus aureus, Pseudomonas species, Burkholderia species, and Candida species.
ADDITIONAL INFORMATION: Burkholderia cepacia is identified in the Clinical Microbiology Laboratory. The isolate will be forwarded to the CF Foundation reference laboratory for confirmation and genotyping. The culture report will not be finalized until the reference laboratory confirmation is complete.