Culture for Neisseria gonorrhoeae Only

CERNER / EPIC MNEMONIC: C GC; C GCGS
POE DESCRIPTION: CU NEISSERIA GONORRHOEAE; CU GC; C GC

CU N GONORRHOEAE & STAIN; CU GC AND STAIN; C GCGS

CPT CODE: 87081 (culture); 87205 (Gram stain)
TEST INFORMATION: Test includes culture for Neisseria gonorrhoeae ONLY. Susceptibility testing is NOT routinely performed.
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Gram stain: 2 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 24 hours. Complete culture reports will be issued after 48-72 hours.
SPECIAL INSTRUCTIONS: Order or requisition must state culture for Neisseria gonorrhoeae.
CONTAINER TYPE:

Sealed sterile containerCopanSwabFemaleCopanSwabMale

The swabs may be ordered through SPD #2105023 (female) and #2105024 (male)

SPECIMEN REQUIREMENTS: Body fluid, discharge, pus, swab of genital lesions, urethral discharge, rectal swab, throat swab, vaginal culture, cervical specimen, eye. A less suitable alternative for inpatient specimens ONLY is inoculated modified Thayer-Martin and chocolate agar media (available from the Clinical Microbiology Laboratory).
REJECTION CRITERIA: Inappropriate specimen container, refrigerated specimen, inappropriate specimen source.
MINIMUM VOLUME: 0.5 mL fluid or one swab
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. NEVER refrigerate.
REFERENCE RANGE: No Neisseria gonorrhoeae isolated
LIMITATIONS OF TEST: Screening for Neisseria gonorrhoeae ONLY; no other organsisms will be identified. Overgrowth by other microorganisms may make it impossible to rule out the presence of Neisseria gonorrhoeae.
METHODOLOGY: Conventional culture utilizing media specifically formulated for isolation of N. gonorrhoeae.
ADDITIONAL INFORMATION: A Gram stain smear has a high sensitivity and specificity in a symptomatic male with urethral discharge (95-99%). Endocervical Gram stain is of little value as the sensitivity is lower (50%) and endemic normal flora have a similar morphologic appearance causing potential false positives. Cervical and/or anal and throat cultures are recommended for women. Cervical cultures have a sensitivity of 80-90%. Although demonstration of gram-negative diplococci in white blood cells in a urethral smear from a symptomatic male is presumptive evidence of gonorrhea and is sufficiently diagnostic to initiate therapy, cultural confirmation should be considered if available. The Gram stain will detect 75% of gonococcal conjunctivitis and 10-20% of gonococcal skin lesions. It is of no value in pharyngitis. Patients from whom N. gonorrhoeae is isolated are reported to the City of Chicago Board of Health. This is mandated by law. A Neisseria gonorrhoeae DNA probe assay is available for rapid detection of this pathogen. Refer to Neisseria gonorrhoeae DNA probe assay for additional information.
TEST SYNONYM(S):Culture for N. gonorrhoeae Only; Culture for GC Only; Gonorrhea Culture; Neisseria gonorrhoeae Culture