Culture for Corynebacterium diphtheriae

CERNER / EPIC MNEMONIC: C DIPHTH
POE DESCRIPTION: CU C DIPHTHERIAE; C DIPHTH
CPT CODE: 87081
TEST INFORMATION: Test includes isolation and identification of Corynebacterium diphtheriae ONLY. Susceptibility testing will NOT be performed.
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Preliminary culture reports are available after 48 hours. Cultures with no growth of C. diphtheriae will be issued after 72 hours. Completion of culture reports from specimens in which C. diphtheriae has been isolated may take at least 4 days after receipt of the specimen.
SPECIAL INSTRUCTIONS: The Microbiology Laboratory MUST be notified prior to specimen collection such that a special isolation medium can be prepared. Order or requisition must specify culture for C. diphtheriae.
CONTAINER TYPE: Copan Swab® II, sterile container, Calgiswab®
COLLECTION: Swab throat lesions, the tonsillar crypts, and the nasopharynx. Separate swabs for throat and nasopharynx specimens are desirable. A flexible calcium alginate swab, Calgiswab®, is recommended for obtaining the nasopharyngeal specimen.
SPECIMEN REQUIREMENTS: Throat, nasopharyngeal
REJECTION CRITERIA: Inappropriate specimen container, inappropriate specimen source
MINIMUM VOLUME: One swab
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE: No C. diphtheriae isolated
LIMITATIONS OF TEST: Cultures should be obtained from the nasopharynx, as well as, the throat, since 20% of positive cultures can be missed when only one site is cultured.
METHODOLOGY: Inoculation of conventional and selective culture media.
ADDITIONAL INFORMATION: Culture will be examined for C. diphtheriae ONLY. Isolates of C. diphtheriae are NOT tested for toxin production.
TEST SYNONYM(S):Corynebacterium diphtheriae Culture; Diphtheria Culture