|CERNER / EPIC MNEMONIC:|
CRYPTO AG (for CSF), BL CRYPTO (BLOOD)
|POE DESCRIPTION:||CRYPTOCOCCAL ANTIGEN; CRYPTO AG|
The test is a Lateral Flow Assay which is an immunochromatographic test system for the detection of Cryptococcal antigen. This test replaces India Ink preparation for cerebrospinal fluid specimens.
CSF: daily, 24 hours, performed STAT; blood: Daily, 0800-1430
STAT, 2 hours after receipt of the specimen in the laboratory; routine, same day if received by 1330.
|SPECIMEN REQUIREMENTS:||Cerebrospinal fluid, blood (serum)|
|REJECTION CRITERIA:||Insufficient quantity of specimen, inappropriate specimen container, hemolyzed blood specimen, bloody cerebrospinal fluid specimen.|
1 mL CSF, 2 mL blood (1 mL serum)
|HANDLING INSTRUCTIONS:||Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.|
|REFERENCE RANGE:||No cryptococcal antigen detected|
|LIMITATIONS OF TEST:||
Lateral Flow Assay
A titer will be reported for all positive results. A higher titer usually corresponds with worse disease. Pretreatment of blood specimens with pronase reduces false-positives and increases the sensitivity of the method. A fungal culture should also be performed for patients who are suspected of having cryptococcosis. Antigen detection may precede the detection of viable organisms in cerebrospinal fluid Gram stains and fungal culture. The India Ink preparation has been replaced by the cryptococcal antigen procedure due to the increased sensitivity. A cryptococcal antigen will routinely be performed on all cerebrospinal fluid specimens submitted for a fungal culture providing an adequate volume of specimen is submitted.