Clostridium difficile toxin by PCR

CERNER / EPIC MNEMONIC:

TOXIN CDIF

POE DESCRIPTION:

TOXIN CDIF

CPT CODE:

87493

CDM NUMBER:

3481117

TEST INFORMATION:

Test replaces Clostridium difficile Toxin EIA

DAYS PERFORMED:

Daily

TURNAROUND TIME:

Less than 10 hours

SPECIAL INSTRUCTIONS:

Test not performed on formed stools.  Test not performed on patients less than 2 years of age.  Only one specimen will be tested within a seven day period.

CONTAINER TYPE:

Screw top clean container or white screw top vial included in stool ParaPak collection kit.

COLLECTION:

The specimen should be collected directly into the clean container (no preservative) or into a bedpan, avoiding contamination with urine or water.  Transfer the feces from the bedpan into a clean screw top container.

SPECIMEN REQUIREMENTS:

Fresh unformed stool

REJECTION CRITERIA:

Inappropriate specimen container, insufficient specimen volume, specimen contaminated with urine and/or water, rectal swab, preserved specimen, specimen submitted within the past week, formed stool specimen, child less than two years old, unlabeled or inappropriatedly labeled specimen.

MINIMUM VOLUME:

5 ml or 1 gram

HANDLING INSTRUCTIONS:

Send to the laboratory as soon as possible.  Keep at room temperature.

REFERENCE RANGE:

No Clostridium difficile toxin detected.

LIMITATIONS OF TEST:

Inhibition of the Assay has been observed in the presence of zinc oxide paste and Vagisil cream.

METHODOLOGY:

Real Time PCR

ADDITIONAL INFORMATION:

Contact precautions is required for all patients with diarrhea due to Clostridium difficile for the duration of their illness.

LAST UPDATED:

02-21-12

TEST SYNONYM(S):Clostridium difficile Assay; Clostridium difficile Toxin Test