|CERNER / EPIC MNEMONIC:||C CHLAM|
|POE DESCRIPTION:||CU CHLAMYDIA; C CHLAM|
Obtain the viral transport medium from the Clinical Microbiology laboratory. Wipe the epithelial cell surface to remove any excess mucus. Discard the swab. Using a second swab, vigorously swab the site to be cultured and place the swab directly into the viral transport medium. Place tissue and/or aspirate specimens directly into the viral transport medium. Specimens should be collected early in the acute phase of the infection first 3-5 days after onset.
Conjunctival swab, posterior nasopharyngeal aspirate, cervical swab, urethral swab, tissue, lymph node aspirate, rectal swab.
|HANDLING INSTRUCTIONS:||Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.|
|REFERENCE RANGE:||No Chlamydia isolated|
|LIMITATIONS OF TEST:||
|METHODOLOGY:||Tissue culture and direct fluorescent antibody (DFA).|
|ADDITIONAL INFORMATION:||Chlamydia infects the columnar epithelical cells and will not be recovered from squamous epithelial cells or inflammatory cells. In obtaining the specimen, clean the area of inflammatory cells and then attempt to scrape epithelial cells for culturing. Patients in whom Chlamydia trachomatis is isolated are reported to the City of Chicago Board of Health. This is mandated by law.|
|TEST SYNONYM(S):||Lymphogranuloma Venereum Culture|