Brucella Antibody Panel

CERNER / EPIC MNEMONIC:

BRUCELLA

POE DESCRIPTION: MICROBIOLOGY REFERRAL
CPT CODE: 86622 (x2)
TEST INFORMATION: Test includes antibody titers to Brucella abortus.
DAYS PERFORMED: Monday-Friday, 0700-1400
TURNAROUND TIME: 2-5 days
SPECIAL INSTRUCTIONS: Acute and convalescent specimens should be submitted with the convalescent specimen submitted 2-3 weeks after the acute. It is the requesting physician`s responsibility to see that both acute and convalescent specimens are obtained.
CONTAINER TYPE:

Red top Vacutainer® tube or SSTTM tube, sterile container with no preservative for CSF

SPECIMEN REQUIREMENTS:

Blood (serum), CSF

REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume.
MINIMUM VOLUME:

5 mL blood (1 mL serum) or 1 mL CSF

HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
REFERENCE RANGE:

Serum:  < .80

CSF   :  < .50

LIMITATIONS OF TEST: Cross reactions can occur with other Brucella species, Yersinia enterocolitica serotype 9, Francisella tularensis, and Vibrio cholerae. Brucella abortus antigen detects infection also caused by B. suis and B. melitensis, but not B. canis.
METHODOLOGY:

ELISA

REFERRAL LABORATORY: Commercial Laboratory
ADDITIONAL INFORMATION:

Antibody titers are measured against Brucella abortus ONLY. Brucellosis antibody appears during the second week in acute cases and peaks in 3-6 weeks. Acute disease is manifested by the presence of specific IgM and increasing amounts of IgG antibodies. Elevated concentrations of Brucella specific IgG are seen in patients with chronic brucellosis.

LAST UPDATED:

5-19-2014