Bronchial Aspirate Culture, Fungus

CPT CODE: 87102 (culture); 87206 (smear)
TEST INFORMATION: Test includes a culture for fungi and performance of a direct fungal smear. All fungal isolates will be identified. Other isolated organisms (i.e. aerobes, mycobacteria) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Smear results: same day if the specimen is received in the laboratory no later than 1400. Preliminary culture reports are available after 1 week or when a fungal isolate is detected. Cultures will be finalized after 4 weeks.
SPECIAL INSTRUCTIONS: The specific anatomic site of the specimen MUST be specified on the order or requisition.
CONTAINER TYPE: Sealed sterile container, no preservative; sterile sputum container; sterile Leuken`s tube
COLLECTION: Specimens are to be collected from a site prepared utilizing aseptic technique. Non-bacteriostatic sterile saline should be used when obtaining washings and/or lavage specimens. Material obtained by needle aspiration (transbronchial lung biopsy) is injected into a sealed sterile container.
SPECIMEN REQUIREMENTS: Bronchial aspirate, bronchial biopsy, bronchial brush, endotracheal aspirate, transtracheal aspirate, tracheal aspirate
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative.
MINIMUM VOLUME: 1 mL or a piece of tissue
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate.
REFERENCE RANGE: No fungi observed/isolated.
LIMITATIONS OF TEST: Anesthetic solutions used during the bronchoscopy procedure may inhibit growth of organisms. Bronchial brushes MUST be transported to the laboratory IMMEDIATELY to eliminate dessication of the specimen. Specimens collected via bronchoscopy are contaminated with normal oral flora.
METHODOLOGY: Fungal smear: calcofluor white stain. Culture: Conventional culture utilizing media specifically formulated for fungal isolation.
ADDITIONAL INFORMATION: Please notify the Clinical Microbiology Laboratory (312-942-5452) if Histoplasma capsulatum, Blastomyces dermatitidis, or Coccidioides immitis is suspected. These pathogens are highly infectious when propagated in vitro and have been transmitted to exposed laboratory workers.
TEST SYNONYM(S):Fungus Culture, Bronchial Aspirate