Bordetella pertussis PCR Performed at IDPH

CERNER / EPIC MNEMONIC: MICRO REF
POE DESCRIPTION: MICROBIOLOGY REFERRAL
CPT CODE: 87265 - No Fee
TEST INFORMATION: Test includes fluorescent antibody smear for specified organism.
DAYS PERFORMED: Monday-Friday, 0700-1400
TURNAROUND TIME: 2-5 days
SPECIAL INSTRUCTIONS: Requisition must specify Bordetella pertussis and/or B. parapertussis.  The specific anatomic site of the specimen MUST be specified on the order or requisition.
CONTAINER TYPE: Transport medium; sealed sterile container.
COLLECTION: Shape the flexible swab into the contour of the nares and into the nasopharynx. Pass the swab gently through the nose. Leave the swab in place near the septum and floor of nose for 15-30 seconds. Rotate and remove. Insert the swab into the transport medium. Nasopharyngeal washings or aspirate are preferred. Submit to the Clinical Microbiology laboratory IMMEDIATELY.
SPECIMEN REQUIREMENTS: Calgiswab® nasopharyngeal (NP) swab; nasopharyngeal washings (sealed sterile container).
REJECTION CRITERIA: Swab specimen not submitted in transport medium; insufficient quantity.
MINIMUM VOLUME: One NP swab; 1 mL washings/aspirate
HANDLING INSTRUCTIONS: When transportation MUST be delayed, leave at room temperature.
REFERENCE RANGE: No B. pertussis and/or B. parapertussis observed
LIMITATIONS OF TEST:

PCR test does not differentiate live organisms from dead.

METHODOLOGY:

PCR

REFERRAL LABORATORY: IDPH Laboratories
ADDITIONAL INFORMATION: Culture for Bordetella pertussis.
TEST SYNONYM(S):Bordetella parapertussis PCR