Bone Marrow Culture, Mycobacterium

CERNER / EPIC MNEMONIC:

C AFB BL

POE DESCRIPTION: CU MYCOBACTERIUM W SMEAR; CU AFB W SMEAR; C AFBSM
CPT CODE:

87116 (culture)

TEST INFORMATION:

Culture only, no acid fast stain (if specimen volume is adequate). If a mycobacterial species is isolated, the organism will be identified. Susceptibility testing will be performed depending upon the isolate identification. Other isolated organisms (i.e. aerobes, fungi) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).

DAYS PERFORMED: Daily
TURNAROUND TIME:

Preliminary culture reports are available after 1 week or when a mycobacterial isolate is detected. Negative cultures will be finalized after 8 weeks. Completion of culture reports may require additional time when mycobacteria are isolated and/or susceptibility testing is performed.

CONTAINER TYPE:

Bactec Myco/F Lytic bottle (available from Clinical Microbiology, 312-942-5452)

COLLECTION:

Specimens are to be collected from a site prepared utilizing aseptic technique. Material obtained by needle aspiration, is injected into the bottle. Invert the tube 5-10 times to mix well.

SPECIMEN REQUIREMENTS: Bone marrow
REJECTION CRITERIA:

Inappropriate specimen container, insufficient specimen volume, refrigerated specimen.

MINIMUM VOLUME: 0.5 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate.
REFERENCE RANGE:

No acid-fast bacilli isolated.

METHODOLOGY:

Culture:  Automated continuous analysis

ADDITIONAL INFORMATION:

DNA probes may be incorporated to aid with more rapid identification of mycobacterial isolates. This will generate an additional charge(s). Susceptibility testing will be performed only on the first Mycobacterium isolate from each specimen type per patient. Additional susceptibilities may be performed after a period of 4 weeks for patients with suspected treatment failure. Susceptibility testing is performed routinely only for M. tuberculosis complex.  Susceptibility testing for other Mycobacterium species is performed by special request only. The battery of drugs routinely tested will vary by isolate and/or physician request. Patients from whom M. tuberculosis is isolated in culture are reported to the City of Chicago Board of Health. This is mandated by law.

LAST UPDATED:

5-13-2014

TEST SYNONYM(S):AFB Culture, Bone Marrow; Mycobacterium Culture, Bone Marrow; TB Culture, Bone Marrow