Body Fluid Culture, Fungus

CERNER / EPIC MNEMONIC: C FUNGUSSM; C FUNGUS (CSF only)
POE DESCRIPTION: CU FUNGUS W SMEAR (NON-SKIN); C FUNGUSSM

For CSF only: CU FUNGUS (NON-SKIN); C FUNGUS (NON-SKIN)

CPT CODE: 87102 (culture); 87206 (smear)
TEST INFORMATION: Test includes a culture for fungi and performance of a direct fungal smear. All fungal isolates will be identified. A Cryptococcal Antigen test will routinely be performed on all cerebrospinal specimens in lieu of a fungal smear if an adequate volume of sample is submitted. Other isolated organisms (i.e. aerobes, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Smear results: same day if the specimen is received in the laboratory no later than 1400. Preliminary culture reports are available after 1 week or when an isolate is detected. Cultures will be finalized after 4 weeks.
SPECIAL INSTRUCTIONS: The specific anatomic site of the body fluid MUST be specified on the order or requisition.
CONTAINER TYPE: Sealed sterile container, no preservative; Copan Swab® II
COLLECTION: The specimen of choice is an aspirate, NOT a swab. Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) organisms. Ideally, material obtained by needle aspiration through intact surface which has been cleaned with antiseptic, is put directly into a sealed, sterile container. Swabs should be used as a last resort due to the small volume of specimen obtainable by this method. If a swab must be used, use the Copan Swab® II collection device. Contamination with normal flora from skin or other body surfaces must be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
SPECIMEN REQUIREMENTS: Aspirated body fluid
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, specimen submitted in a preservative.
MINIMUM VOLUME: 0.5 mL
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE: No fungi isolated/observed.
METHODOLOGY: Fungal smear: calcofluor white stain. Culture: Conventional culture utilizing media specifically formulated for fungal isolation.
TEST SYNONYM(S):Fungus Culture, Body Fluid