Body Fluid Culture, Anaerobic

CERNER / EPIC MNEMONIC: C ANAEROBE
POE DESCRIPTION: CU ANAEROBIC; C ANAEROBE
CPT CODE: 87075
TEST INFORMATION: Test includes culture for anaerobic organisms. Other isolated organisms (i.e. aerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours
TURNAROUND TIME: Cultures are examined every 48 hours. Preliminary reports available after 48 hours. Culture reports of no growth will be issued after 5 days. Completion of culture reports may take 7-10 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.
SPECIAL INSTRUCTIONS: Avoid exposure to atmospheric oxygen. Specimens should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. The specific anatomic site of the body fluid MUST be specified on the order or requisition.
CONTAINER TYPE: BBLTM Port-A-Cul Transport System; Bact/Alert® SN (anaerobic) bottles
COLLECTION: The specimen of choice is an aspirate, NOT a swab. Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) anaerobes. Ideally, material obtained by needle aspiration through intact surface which has been cleaned with antiseptic, is put directly into either the BBLTM Port-A-Cul Transport System or 0.5-10 mL into the Bact/Alert® SN (anaerobic) bottles. If Bact/Alert® SN bottles are not directly inoculated, all air should be expelled from the syringe after which the specimen should be injected into the BBLTM Port-A-Cul Transport System. Swabs should be used as a last resort due to the small volume of specimen obtainable by this method. If a swab must be used, insert the swab containing the specimen about 1 cm from the bottom of the transport tube. Break off the swab shaft and secure the lid tightly. Contamination with normal flora from skin or other body surfaces MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
SPECIMEN REQUIREMENTS: Aspirated body fluid, pleural, peritoneal, pericardial, synovial, and other normally sterile sites and fluids
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, gross contamination of specimen. Specimens from sites which have anaerobic bacteria as normal flora will routinely be rejected.
MINIMUM VOLUME: 0.5-10 mL; 0.5-10 mL per Bact/Alert® SN (anaerobic) bottles
HANDLING INSTRUCTIONS: Avoid exposure to atmospheric oxygen. Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. Clients: incubate Bact/Alert® SN bottles if transportation is delayed more than 4 hours at 35­°C.
REFERENCE RANGE: No growth of anaerobic bacteria.
LIMITATIONS OF TEST: A specimen received in anaerobic transport devices is not suitable for routine fungal or mycobacterial cultures. If more than three anaerobic isolates are present, the Bacteroides fragilis group, pigmented gram-negative rods (Prevotella and/or Porphyromonas species), Actinomyces species, and Clostridium perfringens will be identified. Anaerobic susceptibility testing is not performed routinely. Should susceptibility testing be required, call the Clinical Microbiology laboratory at (312)942-5452.
METHODOLOGY: Conventional culture utilizing media specifically formulated for anaerobic isolation or automation (continuous monitoring) for Bact/Alert® SN bottles.
ADDITIONAL INFORMATION: The use of blood culture bottles for submission of the specimens has some limitations: quantitation unavailable, Gram stain unavailable unless a separate sample is submitted. Use of the bottles does however increase the sensitivity of the culture procedure and provides for automated continuous monitoring.
TEST SYNONYM(S):Anaerobic Culture, Body Fluid