Biopsy Culture, Fungus

CPT CODE: 87102 (culture); 87206 (smear)
TEST INFORMATION: Test includes a culture for fungi and performance of a direct fungal smear. All fungal isolates will be identified. Other isolated organisms (i.e. aerobes, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
DAYS PERFORMED: Daily, 24 hours.
TURNAROUND TIME: Smear results: same day if the specimen is received in the laboratory no later than 1400. Preliminary culture reports are available after 1 week or when a fungal isolate is detected. Cultures will be finalized after 4 weeks.
SPECIAL INSTRUCTIONS: The specific anatomic site of the biopsy MUST be specified on the order or requisition with surface and/or deep or surgical biopsies delineated.
CONTAINER TYPE: Sealed sterile container, no preservative; sterile Petri dish (available from the Clinical Microbiology Laboratory).
COLLECTION: Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface organisms. Ideally, material is obtained by needle aspiration through intact surface, which has been cleaned with antiseptic, and injected into a sealed, sterile container. Sampling of open lesions is enhanced by deep aspiration using a sterile plastic catheter. If irrigation is necessary, nonbacteriostatic sterile saline may be used. Pulmonary samples may be obtained by transtracheal percutaneous needle aspiration. Optimal isolation of fungi from tissue is accomplished by processing as much tissue as possible.
SPECIMEN REQUIREMENTS: Surgical tissue, biopsy material
REJECTION CRITERIA: Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, specimen received in a preservative.
MINIMUM VOLUME: As much as possible
HANDLING INSTRUCTIONS: Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
REFERENCE RANGE: No fungi isolated/observed.
METHODOLOGY: Fungal smear: calcofluor white stain. Culture: Conventional culture utilizing media specifically formulated for fungal isolation.
ADDITIONAL INFORMATION: The portion of the surgical specimen submitted to the Clinical Microbiology Laboratory should be aseptically separated from the portion submitted to the Pathology Department.
TEST SYNONYM(S):Fungus Culture, Biopsy