Culture for Acanthamoeba

CPT CODE: 87999
TEST INFORMATION: Test includes culture for Acanthamoeba species only.
DAYS PERFORMED: Mon-Fri, 0800-1600
TURNAROUND TIME: Preliminary reports are available after 48 hours or when ameba are detected. Cultures will be finalized after 1 week.
SPECIAL INSTRUCTIONS: The Clinical Microbiology Laboratory MUST be contacted at (312) 942-5452, prior to submission of the specimen. The specific anatomic site of the specimen MUST be specified on the order or the requisition.
CONTAINER TYPE: Sealed, sterile container
COLLECTION: The specimens of choice are cerebrospinal fluid and ocular specimens. Specimens are to be collected from a site prepared utilizing aseptic technique. Swabs must NOT be used. Contamination with normal flora from skin or other body sources MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
SPECIMEN REQUIREMENTS: Cerebrospinal fluid, corneal biopsy, corneal scraping, contact lens cleaning solution/drops
REJECTION CRITERIA: Specimen received in a fixative.
MINIMUM VOLUME: 0.5 mL or piece of tissue
HANDLING INSTRUCTIONS: Specimen MUST be transported directly to the Clinical Microbiology Laboratory, 1133 Jelke SC as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate.
REFERENCE RANGE: No parasites found
METHODOLOGY: Culture in non-nutrient agar with Esherichia coli.
ADDITIONAL INFORMATION: Naegleria species cause primary amebic meningoencephalitis. Acanthamoeba species cause disseminated infection in severely immunocompromised individuals, chronic granulomatous amebic encephalitis and/or sight-threatening ocular infections.
TEST SYNONYM(S):Naegleria species; Free Living Amebas