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CERNER / EPIC MNEMONIC / CPT CODE / CDM NUMBER / TEST INFORMATION / DAYS PERFORMED / TURNAROUND TIME / CONTAINER TYPE / SPECIMEN REQUIREMENTS / REJECTION CRITERIA / MINIMUM VOLUME / HANDLING INSTRUCTIONS / REFERENCE RANGE / LIMITATIONS OF TEST / METHODOLOGY / REFERRAL LABORATORY / TEST SYNONYM(S)

Blastomyces Dermatitidis Quantitative Antigen EIA (View in report form.)

CERNER / EPIC MNEMONIC:

BLASTO AG

CPT CODE:

87449

CDM NUMBER:

3181502

TEST INFORMATION:

Intended use is as an aid in diagnosis of  blastomycosis or to monitor therapy.

DAYS PERFORMED:

Test performed  Monday - Friday only.

TURNAROUND TIME:

2 - 7 days

CONTAINER TYPE:

Sealed sterile urine container (no preservative); red top Vacutainer tube or SST tube; sealed sterile cerebrospinal fluid collection tube; sealed sterile sputum or Leuken's container.

SPECIMEN REQUIREMENTS:

Urine, serum separated from the clot, plasma separated from the red cell layer, CSF, bronchial, or other sterile body fluid

REJECTION CRITERIA:

Inadequate specimen volume, particulate matter or viscosity that would not allow the specimen to be pipetted.

MINIMUM VOLUME:

2.0 mL

HANDLING INSTRUCTIONS:

Store in refrigerator if not sent the same day as obtained.

REFERENCE RANGE:

Negative = None Detected, Low Positive = Below the Limit of Quantification,  Low Positive = 0.20-1.99 ng/ml, Moderate Positive = 2.00-14.70 ng/ml, High Positive = Above the Limit of Quantification

LIMITATIONS OF TEST:

Cross-reactions occur in histoplasmosis, paracoccidioidomycosis, penicilliosis, coccidioidomycosis and rarely in aspergillosis and possibly in sporotrichosis.  Sputolysin and NaOH treatment degrade the analyte detected in the assay.  Negative results do not exclude blastomycosis: testing both urine and serum offers the highest sensitivity.

METHODOLOGY:

Quantitative sandwich enzyme immunoassay (EIA)

REFERRAL LABORATORY:

Commercial Laboratory


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