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CERNER / EPIC MNEMONIC / POE DESCRIPTION / CPT CODE / CDM NUMBER / TEST INFORMATION / DAYS PERFORMED / TURNAROUND TIME / SPECIAL INSTRUCTIONS / CONTAINER TYPE / SPECIMEN REQUIREMENTS / REJECTION CRITERIA / MINIMUM VOLUME / HANDLING INSTRUCTIONS / REFERENCE RANGE / METHODOLOGY / TEST SYNONYM(S)

Mycobacterium Tuberculosis Direct Test (View in report form.)

CERNER / EPIC MNEMONIC: AFB MTB
POE DESCRIPTION: AFB MTB
CPT CODE: 87556
CDM NUMBER: 3421131
TEST INFORMATION:

Detection of presence (positive) or lack of presence (negative) of Mycobacterium tuberculosis complex rRNA.

[Note:¿ The Gen-Probe¿ amplification system (MTD) is specific for, but does not differentiate among, members of the M. tuberculosis complex.]

DAYS PERFORMED:

Monday, Wednesday, Friday

TURNAROUND TIME:

1-5 days

SPECIAL INSTRUCTIONS: Complete IDPH requisition providing code, patient's name, specimen source, date of collection.¿ Notify IDPH laboratory of specimen arrival.
CONTAINER TYPE: Sealed sterile container, no preservative
SPECIMEN REQUIREMENTS: Undigested sputum (induced or expectorated), Bronchial specimens (bronchoalveolar lavage or bronchial aspirate), tracheal aspirate.
REJECTION CRITERIA:

1. Specimen without submission form.

2. Incomplete/inaccurate information on submission¿form.

3. Specimen other than sputum (induced or expectorated), bronchial¿specimens (bronchoalveolar lavages or bronchial aspirates), tracheal¿aspirates or

4. Specimen from previous MTD test "positive" (without written justification¿from physician).

5. Patient specimens with a 'negative' MTD result will only be tested three¿times in a 90-day period.

6. Specimens not stored at 2¿ to 8¿C before transport.

7. Specimens older than 72 hrs.

8. Specimen not labeled.

9. Grossly bloody specimen.

MINIMUM VOLUME: 1.5 mL
HANDLING INSTRUCTIONS: Specimen should be frozen as soon as possible.¿ Specimen should be transported frozen with an ice pack.
REFERENCE RANGE: Not detected.
METHODOLOGY: The Gen-Probe¿ amplification system amplifies a specific ribosomal RNA (rRNA) target via DNA intermediates.¿ Detection of the amplified rRNA sequence (amplicon) is achieved using nucleic acid hybridization.¿ The chemiluminescent single-stranded DNA probe is complementary to the amplicon.¿ The labeled DNA probe combines with the amplified ribosomal RNA to form a stable RNA:DNA hybrid.¿ The selection reagent differentiates hybridized from non-hybridized probe.¿ The labeled RNA:DNA hybrids are measured in a luminometer.
TEST SYNONYM(S):Mybacterium Tuberculosis Direct Test, Amplified (MTD

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