Biopsy, bone marrow, blood, body fluid. Note: Leftover specimen submitted to Pathology or Cellular Laboratory will be used whenever possible. Specimens submitted for the Lymphoma and Leukemia Gene Rearrangement test will be used to determine the presence of an amplifiable bc1-2 translocation.
The bcl-2 translocation test by PCR may be ordered alone; in this case the sample quantity required is 2-3 mL of blood and 1-2 mL of bone marrow.
Store yellow top (ACD) tubes at room temperature; deliver to the Cellular Laboratory within 48 hours; 24 hours for quantitative PCR
No translocation observed; 0.01-100% of cells in specimen.
LIMITATIONS OF TEST:
Will detect 80% of translocation breakpoints
bc1-2 Translocation; Major Breakpopint Region (MBR) Translocation; Minor Cluster Region (MCR)