Test includes a culture for fungi and performance of a direct fungal smear. All fungal isolates will be identified. Other isolated organisms (i.e. aerobes, mycobacteria, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
Daily, 24 hours. Cultures are examined Monday-Saturday.
Smear results: same day if the specimen is received in the laboratory no later than 1400. Preliminary culture reports are available after 1 week or when a fungal isolate is detected. Culture reports will be finalized after 4 weeks.
The specific anatomic site of the specimen MUST be specified on the requisition.
The specimen of choice is an aspirate or tissue, NOT a swab. Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) organisms. Ideally, material is obtained by needle aspiration through an intact surface, which has been cleaned with antiseptic. Sampling of open lesions is enhanced by deep aspiration using a sterile plastic catheter. Curetting of the base of an open lesion is optimal. If irrigation is necessary, nonbacteriostatic sterile saline may be used. When a syringe is used to obtain the specimen, ALL air should be expelled after which the specimen should be injected into a sealed sterile container. Swabs should be used as a last resort due to the small volume of specimen obtainable by this method. If a swab must be used, sample the advancing margin of the lesion and abscess walls firmly. Do NOT sample pus or exudate ONLY. Contamination with normal flora from skin or other body sources MUST be avoided since colonizing bacteria and/or saprophytic fungi not involved in the infectious process may be introduced into the sample.
Pus or other purulent fluid material appropriately obtained from a wound
Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative.
0.5 mL or two swabs
Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
No fungi observed/isolated
LIMITATIONS OF TEST:
The Calcofluor white stain demonstrates yeast forms, spores, and the hyphae of fungi. The filaments of Nocardia species, Streptomyces species, and Actinomyces species, as well as, the capsule of Cryptococcus species are NOT satisfactorily demonstrated with this methodology.
Fungal smear: calcofluor white stain. Culture: Conventional culture utilizing media specifically formulated for fungal isolation.