Suprapubic Puncture Anaerobic Culture, Urine (View in report form.)
CERNER / EPIC MNEMONIC:
Test includes culture for anaerobic organisms. Other isolated organisms (i.e. aerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
Daily, 24 hours
Cultures are examined every 48 hours. Preliminary culture reports are available after 48 hours. Culture reports of no growth will be issued after 6 days. Completion of culture reports may take 7-10 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.
Avoid exposure to atmospheric oxygen. Specimens should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. The specific source of the urine specimen MUST be specified on the requisition.
BBL Port-A-Cul Transport System
The specimen of choice is an aspirate, NOT a swab. Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) anaerobes. Ideally, material is obtained by needle aspiration through an intact surface, which has been cleaned with antiseptic, then placed directly into the BBL Port-A-Cul Transport System. When a syringe is used to obtain the specimen, ALL air should be expelled after which the specimen should be injected into a BBL Port-A-Cul Transport System. Contamination with normal flora from skin or other body sources MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
Urine obtained by suprapubic puncture
Inappropriate specimen container, insufficient specimen volume, refrigerated specimen. Specimens from sites which have anaerobic bacteria as normal flora will routinely be rejected (i.e. voided. catheterized urine specimens).
Avoid exposure to atmospheric oxygen. Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate.
No growth of anaerobic bacteria
LIMITATIONS OF TEST:
A specimen received in anaerobic transport devices is not suitable for routine fungal or mycobacterial cultures. If more than three anaerobic isolates are present, the Bacteroides fragilis group, pigmented gram-negative rods (Prevotella and/or Porphyromonas species), and Clostridium perfringens will be the only isolates identified. Anaerobic susceptibility testing is NOT performed routinely. Should susceptibility testing be required, call the laboratory.
Conventional culture utilizing media specifically formulated for anaerobic isolation.