Respiratory Syncytial Virus Culture and Antigen (View in report form.)
CERNER / EPIC MNEMONIC:
RSV AG; C VIR
RSV ANTIGEN DETECTION
The test includes a direct screening test for RSV antigen. A viral culture is perfomed on all RSV antigen screens.
Monday-Saturday, 0700-1400. During respiratory viral season, the assay is perfomed Monday-Friday at 0830, 1330, 1930, and 0230; Saturday and Sunday at 0230, 1330, and 1930.
Antigen detection: same day providing the specimen is received by 1330 or same day during respiratory viral season. Culture: 7 days.
Viral transport medium
Introduce 3-7 cc sterile saline into the patient's posterior nasal cavity and immediately aspirate the fluid via vacuum suction and Leuken's trap. Specimens must then be placed into the viral transport medium (available from the Clinical Microbiology Laboratory).
Nasopharyngeal washes and secretions
Inappropriate specimen container, specimen NOT submitted in viral transport medium, specimen NOT refrigerated during transport, excessive delay in transport.
Specimen should be transported as soon as possible. When transportation is delayed, refrigerate. Keep specimen cold and moist. Do NOT freeze specimen.
No RSV antigen detected; No virus isolated.
LIMITATIONS OF TEST:
Respiratory syncytial virus is extremely labile.
Direct antigen detection: Enzyme-linked immunosorbent assay (ELISA).Culture: Inoculation of specimens into cell cultures, incubation, observation for characteristic cytopathic effect in 2-7 days, and identification by fluorescent monoclonal antibodies specific for Respiratory Syncytial Virus.
Serology is also available to detect antibodies to Respiratory Syncytial Virus.