Parainfluenza 1, 2, 3 Virus Culture (View in report form.)
CERNER / EPIC MNEMONIC:
CU VIRUS; C VIR
The test includes concurrent culture for other respiratory viruses (i.e. Influenza, Adenovirus, and Respiratory Syncytial Virus).
Sealed sterile container, viral transport medium
Infants and small children: soft catheters and suction devices (syringes and suction bulbs) can be used to collect nasal secretions (the optimal specimen) as the catheter is withdrawn from far back in the nose. The collection method of choice is to introduce 3-7 mL of sterile saline into the child`s posterior nasal cavity and immediately aspirate the fluid. Specimens MUST then be placed into the viral transport medium (available from the Clinical Microbiology Laboratory).
Throat washes, nasopharyngeal washes and secretions
Inappropriate specimen container, specimen NOT submitted in viral transport medium, specimen NOT refrigerated during transport, excessive delay in transport.
Specimen should be transported as soon as possible. When transportation is delayed, refrigerate. Keep specimens cold and moist. Do NOT freeze.
No virus isolated
Inoculation of specimens into cell cultures, incubation, observation for hemadsorption or characteristic cytopathic effect, and identification/speciation by fluorescent monoclonal antibodies specific for Parainfluenza types 1, 2, or 3.
The direct fluorescent monoclonal antibody method allows specific typing of the Parainfluenza virus.