Culture for Legionella pneumophila (View in report form.)
CERNER / EPIC MNEMONIC:
CU LEGIONELLA AND SMEAR; C LEGIONSM
87077; 87278; 87081
The test includes culture and direct fluorescent antibody (DFA) smear for Legionella pneumophila.
Daily, 24 hours
DFA smear results are available Monday-Friday. Specimens received by 0900 will have the DFA results reported the same day. Preliminary cultures reports are available after 48 hours. Culture reports of no growth will be issued after 5 days. Completion of culture reports may take 6-7 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.
Specimens are to be collected from a site prepared utilizing aseptic technique. Non-bacteriostatic sterile saline should be used when obtaining washings and/or lavage specimens. Material obtained by needle aspiration (transbronchial lung biopsy) is injected into a sealed sterile container.
Lung tissue, other body tissue, pleural fluid, other body fluid, transtracheal aspiration, bronchoalveolar lavage, BAL, and bronchial brushing.
Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
DFA: No Legionella pneumophila detected
Culture: No Legionella species isolated
LIMITATIONS OF TEST:
The sensitivity of the DFA smear is relatively low (25-70%); however, the specificity is >95%. Not all serogroups of Legionella pneumophila are detected by DFA. The sensitivity of the culture is 50-80%; however, the specificity is 100%.
Smear: direct fluorescent antibody.
Culture: conventional culture using routine media and media selective for Legionella isolation.
If clinical suspicion of Legionella is high, several specimens may need to be submitted since small numbers of organisms may be present. Legionella species are widespread in the environment and are associated almost exclusively with surface and potable waters or moist environments. Click here for more information about Legionnaire's disease.