The test includes a KOH preparation ONLY. A fungal culture will NOT be performed unless specifically requested.
Daily, 800 - 1600
2 hours after receipt of the specimen in the laboratory
Sealed sterile container, no preservative; sterile Petri dish (available from Clinical Microbiology Laboratory); Copan Swab II
Infected hair should be cut with sterile scissors or plucked with sterile forceps and placed in a sealed, sterile container. A Wood`s lamp is useful in the collection of specimens in tinea capitis infections, since hairs infected by most members of the genus Microsporum exhibit fluorescence under a Wood`s lamp. If diseased hair stubs or fluorescent hairs are not apparent, scrape the edge of a scalp lesion with a sterile scalpel. Infected nails should be sampled from beneath the nail to obtain softened material from the nail bed. If unable to obtain adequate material from the nail bed, a scalpel should be utilized to scrape the superficial portions of the nail obtaining subsurface material. Nail clippings are unacceptable. When obtaining a cutaneous specimen, the area to be sampled should first be cleansed with 70% alcohol to remove bacterial contaminants. Typical 'ringworm' lesions should be sampled from the erythematous, peripheral growing margin.Genital specimens should be collected using aseptic technique.
Hair, nail scrapings, skin scrapings, genital specimens
Inappropriate specimen container, insufficient specimen volume, specimen received in a preservative, nail clippings
As much as possible of hair, nail scrapings, skin scrapings; two genital swabs
Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
No fungal elements observed
LIMITATIONS OF TEST:
The test may require overnight incubation for complete disintegration of keratin. A fungal culture is more sensitive than the KOH preparation; therefore, the KOH Preparation may be negative when the Fungus Culture is positive.