Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
5 mL blood (1 mL serum)
Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
No Hepatitis B surface antibody detected
LIMITATIONS OF TEST:
In those individuals who have been vaccinated with any of the available HBV vaccines, this test could be useful to show response. However, a positive result in this test cannot guarantee adequate protection from HBV infection.
Enzyme immunoassay (EIA)
The presence of HBsAg antibody indicates previous Hepatitis B infection and acquired immunity to HBV. This test can also be used to evaluate possible immunity in individuals who are at increased risks to further exposure to the Hepatitis B virus (i.e. hemodialysis unit personnel, venipuncturists, etc.). Anti-HBs can usually be detected several weeks to several months after HBsAg is no longer found, and usually persists for many years to a lifetime after acute infection has resolved. In an acquired HBV infection, the presence of the antibody without the presence of the HBsAg may indicate immunity from reinfection.
Antibody to Hepatitis B Surface Antigen; Anti-HBs; Hepatitis B Antibody