Excessive hemolysis, gross contamination of specimen, chylous serum, improper storage of specimen, inappropriate specimen container, insufficient specimen volume.
5 mL blood (1 mL serum)
Specimen should be tranported as soon as possible. When transportation is delayed, refrigerate.
No Hepatitis B core IgM detected
LIMITATIONS OF TEST:
This test should NOT be performed for patients known to have chronic Hepatitis B infection.
Anti-HBc IgM can be detected in 100% of patients within the first month of illness. The antibody will persist for a variable time period but 96% of patients will become negative by 6 months and 98% negative by 9-12 months. Therefore, this test can only be used for the diagnosis of acute Hepatitis B and NOT to determine the immune status of a patient. Anti-HBc IgM may also be present in acute Hepatitis B in the absence of HBsAg.